中文摘要：

目的：构建V806M突变型Axin基因真核表达载体pIRES2-EGFP—MT—Axin（V806M），并稳定表达于大鼠神经胶质瘤细胞系C6。方法：用分子克隆技术，构建真核表达载体pIRES2-EGFP—MT—Axin（V806M），经NheⅠ和SmaⅠ双酶切鉴定后，用脂质体法稳定转染神经胶质瘤细胞C6。结果：构建了真核表达载体pIRES2-EGFP—MT—Axin（V806M），稳定转染后，经荧光显微镜和免疫细胞化学染色法检测，可见细胞内有EGFP及Axin的表达。结论：成功构建真核表达载体pIRES2-EGFP—MT—Axin（V806M），并在神经胶质瘤细胞C6中表达，为研究此种突变体Axin是否影响细胞的生物学行为以及是否参与胶质瘤的发生发展奠定了实验基础。

英文摘要：

Objective： To construct the eukaryotic expression vector plRES2 - EGFP - MT - Axin （ V806M ）, and to express mutant Axin in C6 glioma cells. Methods： The eukaryotic expression vector plRES2 - EGFP - Axin was constructed by introducing mutant Axin（V806M） DNA fragment into the sites of Nhe Ⅰ and Sma Ⅰ of plRES2 - EGFP vector. The plasmid was transfected into the C6 cells using hpofectamine. Results： The eukaryotic expression vector plRES2 -EGFP - MT -Axin（V806M） was constructed and transfected successfully into C6 glioma cells. The green fluorescence of EGFP was observed in the plasma and nuclei of transfected cells, and Axin protein was only found in the plasma. Conclusion. The recombinant expression vector plRES2 - EGFP - MT - Axin （ V806M ） was constructed, and the EGFP and Axin gene could be co - expressed in the C6 cells. This study laid a foundation for the further research of the function of mutant Axin in cell growth and tumorigenesis.