中文摘要：

为了扩增1型鸭疫里默氏杆菌铁依赖抑制子基因（RaDtxR）全长序列，对SiteFing—PCR技术做了改良。改良后的SiteFinding—PCR技术更加简单、方便且省时。SiteFinding—PCR结果显示，获得的目的片段大小为937bp，向前延伸了710bp。RaDtxR全长基因序列为654个核苷酸，编码217个氨基酸。生物信息学表明，RaDtxR包含完整的铁离子依赖抑制子超家族保守结构域，在进化分类上更接近于白喉棒状杆菌毒素抑制子DtxR。同源建模表明，RaDtxR氨基酸序列含有典型的FUR／DtxR抑制子的HTH结构。

英文摘要：

SiteFinding-PCR was modified to amplify the full length of iron dependent repressor gene of Remerella anatipestifer, which was more simple, convenient and time-saved. The result of modified SiteFinding-PCR indicated the amplified fragment of interest was 937 bp and extended 710 bp, RaDtxR gene consisited of 654 nucleotide acids and encoded 217 amino acids. Bioinformatics analysis showed that there was a complete iron dependent repressor superfamily conserved construction domian. RaDtxR sequence was closer to the diphtheria toxin repressor （DtxR） from C. diphtheriae in the phylogenesis. The structure of RaDtxR protein modeled online using SWISS-MODEL contained the typical HTH structure of Fur/DtxR proteins reported previously.