中文摘要：

目的探讨人神经营养素-3受体TrkC基因重组腺病毒载体（Adeno—TrkC）在神经干细胞内的表达及其对神经干细胞的生物活性作用。方法用RT-PCR检测Adeno—TrkC感染的293细胞的TrkCmRNA含量。用免疫细胞化学和Western blotting检测Adeno—TrkC感染的神经干细胞表达TrkC受体蛋白。在体外培养条件下，观察人神经营养素-3（NT-3）对感染了Adeno—TrkC的神经干细胞分化为神经元样细胞和星形胶质样细胞的影响。结果在Adeno—TrkC感染的293细胞和神经干细胞中检测到TrkCmRNA和TrkC受体蛋白，且表达产物和其配体NT-3结合后能使体外培养的神经干细胞更多地分化为神经元样细胞，分化率达到55．2％，均高于其他对照组。结论Adeno-TrkC能在神经干细胞内表达TrkC受体蛋白．这种TrkC受体蛋白具有促进神经干细胞分化为神经元样细胞活性的作用，这为进一步应用NT-3和TrkC联合进行基因治疗中枢神经损伤研究奠定了基础。

英文摘要：

Objective To detect the expression and the biological activity of a recombinant adenovirus expression vector carrying human neurotrophin-3 （NT-3） receptor TrkC gene （Adeno-TrkC） in neural stem cells. Methods The expression of TrkC mRNA in 293 cells infected with Adeno-TrkC was detected by RT-PCR, and the expression of TrkC protein in neural stem cells infected by Adeno-TrkC was detected with immunocytochemistry and Western blotting. The effects of human neurotrophin-3 （ NT- 3） on the neural stem cells infected by Adeno-TrkC differentiating into neuron-like cells and astrocyte-like cells in vitro were observed. Results The transcription of TrkC mRNA and the expression of TrkC protein was detected in 293 cells and neural stem cells infected by Adeno-TrkC, This kind of TrkC was able to make more neural stem cells differenting into neuron-like cells in vitro with its ligand NT-3 and the percentage of neuron-like cell＇s differentiation was 55.2% which was higher than that of other control groups. Conclusion TrkC protein is expressed in neural stem cells transfected with Adeno-TrkC and is able to make neural stem cells differenting into neuron-like cells. The present study may provide the basis on gene therapy of central nervous injury using further NT-3 and TrkC.