中文摘要：

利用石墨烯的信号放大作用,结合分子印迹技术和电聚合技术,以苯酚为功能单体,胆固醇（ChO）为模板分子,在石墨烯修饰的玻碳电极表面电聚合能识别胆固醇分子的敏感膜,构建胆固醇分子印迹电化学传感器。采用扫描电镜、循环伏安法、差分脉冲伏安法研究了印迹膜的结构、性能和分子印迹效应。结果表明,此传感器对胆固醇有良好的选择性和较高的灵敏度。胆固醇浓度在8.0×10~（-8）~2.0×10~（-4）mol/L范围内与峰电流的变化呈线性关系,检出限（S/N=3）为5.6×10~（-8）mol/L。将其用于人体血清样品的检测,结果满意。

英文摘要：

An electrochemical sensor was fabricated for determination of cholesterol（ChO） based on the molecularly imprinting and signal amplification of graphene.The sensor was prepared by electropolymerizing phenol on the surface of graphene modified glassy carbon electrode with ChO as template.The structure,properties and molecular imprinting effect of the imprinted membrane were studied by SEM,CV and DPV.The results showed that the sensor had good selectivity and high sensitivity to cholesterol.The calibration graph for the determination of cholesterol was linear in the range of 8.0×10~（-8）-2.0×10~（-4）mol/L,with the detection limit（S/N=3） of 5.6×10~（-8）mol/L.The sensor was applied to the determination of cholesterol in human serum samples with satisfactory results.