中文摘要：

将肝靶向分子甘草次酸偶联至聚乙二醇-聚（乳酸-羟基乙酸）（PEG-PLGA）嵌段共聚物上.以聚乙二醇维生素E（TPGS）为稳定剂,采用溶剂挥发法制备肝靶向纳米粒子,通过核磁共振、红外光谱、激光光散射及透射电镜等方法对共聚物及纳米粒子的理化性质进行表征;运用噻唑蓝（MTT）比色法评价纳米粒子作为药物载体的安全性,并通过荧光显微镜初步考察了纳米粒子与肝癌细胞的亲和能力.结果表明,纳米粒子粒径为128.2 nm,电势为-16.2 mV,在电解质溶液中具有较高的稳定性.细胞实验结果显示,该纳米粒子无明显细胞毒性,且甘草次酸的引入能显著增加肝癌细胞对纳米粒子的摄取几率,显示出其作为肝靶向药物载体的潜在价值.

英文摘要：

The glycyrrhetinic acid-modified PEG-PLGA copolymer was fabricated,and was formed into nanoparticles（NPs） via solvent evaporation method with D-α-tocopheryl poly（ethylene glycol） succinate（TPGS） as a stabilizer.The physicochemical properties of the present system were investigated by NMR,IR,DLS,ζ potential and TEM measurements.The cytotoxicity against hepatoma cells was studied based on MTT assay,and the {affinity} between the cells and the NPs were perliminarily evaluated by fluorescence microscopy.The results show that the NPs are regularly spherical in shape with a hydrodynamic diameter around 128.2 nm,and the ζ {potential} of the NPs is about-16.2 mV.Besides,the NPs exhibit good stability against electrolyte solution.MTT result suggest that the NPs almost have no cytotoxicity on cells.Furthermore,the in vitro cell uptake {result} indicate that GA-modified NPs have a high affinity with hepatoma cells,and can be a promising liver targeted material.