中文摘要：

为了高效提取桑叶活性物质,采用热水浸提桑叶中的多糖和黄酮后,再用乙醇-盐酸溶液进一步提取桑叶生物碱,并通过响应面法优化3类桑叶生物活性物质的联合提取工艺条件。优化后的桑叶多糖和黄酮提取工艺条件为提取时间93 min、提取温度81℃、料液质量浓度0.05 g/mL、提取次数4次,在此条件下实测桑叶多糖和黄酮的提取得率分别为15.574%、2.693%;优化后的桑叶生物碱提取工艺条件为提取时间142 min、料液质量浓度0.04 g/mL、乙醇体积分数26.0%、提取次数3次,在此条件下实测桑叶生物碱的提取得率为0.398%。建立的回归模型拟合性好,可用于预测桑叶活性物质提取得率。

英文摘要：

To obtain active substances from mulberry leaves efficiently,we first extracted polysaccharide and flavones from mulberry leaf by hot water method,and then extracted alkaloid from mulberry leaf using ethanol-hydrochloric acid solution.The combined process for extracting these three active substances from mulberry leaf was optimized by response surface methodology.The optimized condition for extracting polysaccharide and flavones from mulberry leaf was extraction time 93 min,extraction temperature 81 ℃,mass concentration of raw material 0.05 g/mL,and 4 times of extraction.Under this condition,the actual extraction yield of polysaccharide and flavones was 5.574% and 2.693%,respectively.The optimized condition for extracting alkaloid from mulberry leaf was extraction time 142 min,mass concentration of raw material 0.04 g/mL,volume fraction of ethanol 26.0%,and 3 times of extraction.Under this condition,the actual extraction yield of mulberry leaf alkaloid was 0.398%.The result indicated that the established model had good fitting performance,and could be used to predict the extraction yield of active substances from mulberry leaves.