中文摘要：

目的探索脂肪间充质干细胞（ADSC）对M1/M2型巨噬细胞的影响以及ADSC能否促使M1型巨噬细胞向M2型巨噬细胞转化。方法利用脂多糖（LPS）、γ干扰素（IFN-γ）刺激J774.1巨噬细胞24 h诱导为M1型巨噬细胞,利用白细胞介素4（IL-4）刺激J774.1巨噬细胞24 h诱导为M2型巨噬细胞;将M1/M2型巨噬细胞分别与ADSC共培养24 h后,收集巨噬细胞和上清液,用实时定量PCR和ELISA检测巨噬细胞IL-6、肿瘤坏死因子α（TNF-α）、诱导型一氧化氮合酶（i NOS）、CC趋化因子配体2（CCL2）、CD86、精氨酸酶1（Arg1）、甘露糖受体/CD206（MR/CD206）、IL-10、炎症区分子1（found in inflammatory zone 1,FIZZ1）、几丁质酶3样分子3（chitinase 3-like 3,即Ym-1）的变化。结果 ADSC使M1型巨噬细胞分泌的IL-6、TNF-α、i NOS、CCL2、CD86明显减少,Arg1、CD206、IL-10明显增加,且上清液中IL-6和TNF-α含量明显减少,CD206明显增加;使M2型巨噬细胞分泌的IL-6、TNF-α、i NOS、CD86明显减少,Arg1、CD206、FIZZ-1、Ym-1、IL-10明显增加,且上清液中IL-6和TNF-α含量明显减少,CD206明显增加。结论 ADSC抑制M1型巨噬细胞的特异性基因的表达,促进M2型巨噬细胞特异性基因的表达,并使M1型巨噬细胞向M2型巨噬细胞转化。

英文摘要：

Objective To investigate the effects of adipose-derived stem cells（ ADSCs） on M1/M2 macrophages and whether ADSCs are able to promote the polarization from M1 macrophages to M2 macrophages. Methods M1 macrophages were induced from J774. 1 macrophages by 24-hour stimulation of lipopolysaccharide（ LPS） and interferon γ（ IFN-γ）,and M2 macrophages were induced from J774. 1 macrophages by interleukin 4（ IL-4） for another 24 hours. Then M1 / M2 macrophages were separately cultured in the presence of ADSCs for 24 hours. The M1 / M2 macrophages and their corresponding supernatants were collected for further analysis. The expressions of IL-6,tumor necrosis factor α（ TNF-α）,inducible nitric oxide synthase（ i NOS）,CC chemokine ligand 2（ CCL2）,CD86,arginase 1（ Arg1）,mannose receptors / CD206（ MR /CD206）,IL-10,found in inflammatory zone 1（ FIZZ1）,chitinase 3-like 3（ Ym-1） were detected by real-time PCR and ELISA. Results ADSCs significantly decreased the levels of IL-6,TNF-α,i NOS,CCL2 and CD86,and increased the levels of Arg1,CD206 and IL-10 in M1 macrophages. In the supernatant of M1 macrophages,the expressions of IL-6 and TNF-αwere reduced,while those of CD206 were enhanced. In M2 macrophages,ADSCs resulted in down-regulation of IL-6,TNF-α,i NOS,CD86 and up-regulation of Arg1,CD206,FIZZ-1,Ym-1 and IL-10. In the supernatant of M2 macrophages,the expression levels of IL-6 and TNF-α were down-regulated and those of CD206 were up-regulated. Conclusion ADSCs can inhibit the gene expression of M1 macrophages and promote the gene expression of M2 macrophages,as well as mediate the polarization from M1 macrophages to M2 macrophages.