中文摘要：

目的：比较针刺不同腧穴对失眠大鼠脑内增食素mRNA表达的影响。方法：将70 Wistar只大鼠随机分为7组,每组10只。分别为模型对照组、空白对照组、＂神门＂组、＂内关＂组、＂三阴交＂组、＂足三里＂组、＂申脉、照海＂组。大鼠腹腔注射氯苯丙氨酸造模成功后,于次日进行针刺治疗,各治疗组针刺相应腧穴,空白对照组及模型对照组只抓取,不治疗。结果：各针刺组oxrexin mRNA表达与模型对照组比较,差异有统计学意义（P〈0.05）;＂申脉、照海＂组与空白对照组比较,差异无统计学意义（P〉0.05）。结论：针刺各组可使失眠大鼠脑内的Orexin含量降低,对失眠具有良性调节的作用。

英文摘要：

Objective ： To compare mRNA expression of Orexin in Brain Tissue on acupuncture at different acupoints prodding insomnia. Methods：Rats were randomly divided into 7 groups：blank control group （blank group）, model control group （model group）, acupunc- ture ＂Shenmai and Zhaohai＂ group, acupuncture ＂Shenmen＂ group, acupuncture ＂Neiguan＂ group, acupuncture ＂Sanyinjiao＂ group, ＂Zusanli＂ group. The rats＇ model was induced by PCPA peritoneal injection. We examined the Prepro-oerxin in rats＇ brain tissue by sime-quantity mRNA to observe the influence of neuropeptide. Results ： PCPA peritoneal injection can step up the positive expression of Orexin in rats＇ brain by sime-quantity mRNA. ＂Shenmai and Zhaohai＂ group has significant effect difference with model group （P 〈 0. 05 ）. Conclusion：Acupuncture can depress the positive expression of Orexin in rats＇ brain and have satisfactory precaution and thera- peutical effect on hyposomnia.