中文摘要：

目的：观察和比较补肾化痰活血中药对再生障碍性贫血小鼠骨髓造血生长因子SCF mRNA、GM-CSF mRNA表达和骨髓CD34＋的影响。方法：建立再障小鼠模型,各组小鼠分别胃饲生理盐水、补肾化痰活血中药、康力龙和再障生血片,连续喂养14天后,采集骨髓进行单个核细胞培养,应用流式细胞术检测骨髓CD3＋4抗原水平;应用RT-PCR方法检测SCFmRNA、GM-CSF mRNA表达水平。结果：①模型组骨髓CD3＋4抗原检测结果显著低于正常对照组,治疗后各组小鼠骨髓CD3＋4抗原水平明显提高,差异有显著性。②模型组骨髓造血生长因子SCF mRNA、GM-CSF mRNA表达水平均低于正常对照组,治疗后各组小鼠骨髓造血生长因子表达水平均明显提高,差异均有显著性。结论：补肾化痰活血方可能通过增强再障小鼠骨髓造血生长因子GM-CSFmRNA、SCFmRNA表达水平,刺激造血细胞分化成熟,达到调控骨髓造血的目的。

英文摘要：

Objective：Observe the effects of the mRNA level of the hematopoietic growth factors SCF and GM-CSF and the bone marrow CD34^＋on aplastic anemia（AA） mice by using kidney-invigorating, sputum-eliminating and blood-activating drugs. Methods：Aplastic anemia mice model was established. Each mouse was gastrogavaged with the normal NaCl, kidney-invigorating, sputum-eliminating,blood-activating drugs,the stanozol and the Aplastic Blood-Forming Tablet. The bone marrow mononuclear cells were collected on Day 14, and the mRNA level of the SCF and GM-CSF was determined with RT-PCR. The expression level of the bone marrow CD34 was detected by flow cytometry. Results ： ①The expression level of the bone marrow CD34^＋ was lower in AA group than the normal control;And the level of each treated group was significantly higher than those in aplastic anemia groups. All had significant difference. ②The expression level of the bone marrow hemopoietic growth factors （SCF mRNA,GM-CSF mRNA） was lower in AA group than the normal control; And the level of each treated group was significantly higher than those in aplastic anemia groups. All had significant difference. Conclusion：The results suggested that the kidney-invigorating, sputum-eliminating and blood-activating prescription can stimulate the differentiation and maturity of hematopoietic cell to achieve the purpose of controlling bone marrow haematogenesis in AA mice by increasing the expression level of hemopoietic growth factors.