中文摘要：

目的观察X射线全身照射对小鼠脾细胞LAMP-1表达的影响。方法采用流式细胞术检测0．075和2Gy X照射后不同时间（0、2、4、8、16、24和48h）以及用Con A刺激4h后，脾细胞表面表达LAMP-1的阳性细胞数。结果脾细胞表面的LAMP-1在2Gy X射线全身照射后8、16和24h表达明显下降；0．075Gy X射线全身照射后2h显著升高，而48h又下降到低于对照的水平。0．075和2Gy X射线全身照射后再加用Con A（10μg／μl）刺激4h，LAMP-1的表达均增强；但是此时2Gy的效应比0．075Gy更明显。结论LAMP-1参与电离辐射作用下免疫信号的传导，高剂量X射线抑制它的表达，而低剂量X射线在早期对其表达有促进作用，与体内免疫细胞的活性密切相关。Con A促进免疫细胞表面LAMP-1的表达。

英文摘要：

Objective To study the effect of WBI with X rays on the expression of LAMP-1 in the splenocytes of mice. Methods The LAMP-1 positive cells from mouse splenocytes were detected with flow cytometry （FCM） at different times（0,2,4,8,16,24 and 48 h） after WBI with X-rays of 0.075 Gy and 2.0 Gy and after WBI added by Con A stimulation. Results In view of time-course effect, the LAMP-1 expression in the splenocytes was significantly inhibited at 8, 16, 24 h after WBI by 2.0 Gy X-rays while the LAMP-1 expression was markedly enhanced at 2 h and suppressed at 48 h after WBI by 0.075 Gy X-rays. Compared with control group ,the LAMP-1 expression in the splenocytes was significantly up-regulated with Con A stimulation（ 10 μg/μl） after WBI with 0.075 and 2.0 Gy X rays. With Con A added, the effect of 2.0 Gy X rays was greater than that of 0.075 Gy. Conclusions LAMP-1 plays a role in the transduction of immune signals induced by ionizing radiation and is closely correlated with the activity of immune ceils. The high dose irradiation inhibits the LAMP-1 expression while the low dose likely causes the opposite biological effect in the early time. Con A enhances the expression of LAMP-1 on the surface of immune ceils.