目的研究番荔枝科鹰爪花属植物毛叶鹰爪花Artabotrys pilosus枝叶中的化学成分。方法采用硅胶柱色谱、反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等方法进行分离,采用理化分析、NMR谱和MS谱等谱学方法鉴定化合物的结构。结果从毛叶鹰爪花枝叶90%乙醇提取物的醋酸乙酯部位中分离得到了16个化合物,分别鉴定为达玛二烯醇乙酸酯(1)、羽扇豆醇(2)、羽扇豆醇乙酸酯(3)、白桦酸(4)、熊果醇(5)、熊果酸(6)、β-香树脂醇(7)、古柯二醇(8)、木栓醇(9)、木栓酮(10)、α-波菜甾酮(11)、豆甾-7-烯-3β-醇(12)、豆甾-5-烯-3β-醇-7-酮(13)、豆甾-3,6-二酮(14)、豆甾醇(15)和β-谷甾醇(16)。结论化合物15和16为首次从毛叶鹰爪花中分离得到,其他化合物为首次从鹰爪花属植物中分离得到。
Objective To study the chemical constituents from twigs and leaves of Artabotrys pilosus. Methods The chemical constituents of A. pilosus were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties, spectral data, as well as comparisons with the data in literature. Results Sixteen compounds were isolated from the acetic ether fraction of 90% ethanol extract from the twigs and leaves of A. pilosus, and identified as dammaradienylacetate(1), lupeol(2), lupenyl acetate(3), betulinic acid(4), uvaol(5), ursolic acid(6), β-amyrin(7), erythrodiol(8), friedelinol(9), friedelin(10), α-spinasterone(11), stigmast-7-ene-3β-ol(12), stigmast-5-ene-3β-ol-7-one(13), stigmastan-3,6-diketone(14), stigmasterol(15), and β-sitosterol(16). Conclusion All compounds are isolated from A. pilosus for the first time. Except for compounds 15 and 16, other compounds are isolated from the plants of Artabotrys R. Br. for the first time.