中文摘要：

目的利用投射电镜观察C57Bl/6J小鼠耳蜗内毛细胞传入神经突触的形态和结构。方法选择发育成熟的C57Bl/6J小鼠,ABR检测听力正常后获取耳蜗标本,分别经过半薄定位和超薄连续切片观察的方法寻找观察耳蜗内毛细胞传入神经突触的形态和结构,探讨发育成熟状态下小鼠耳蜗内毛细胞突触的形态和结构特点,并进一步分析它们可能出现的区域以及与周围解剖结构的关系。结果在听力正常的C57Bl/6J小鼠的不同耳蜗内毛细胞核下区域可以观察到带状突触结构,包括ribbon、突触囊泡、突触间隙和突触后致密带等典型结构;然而有时即使是在听力正常的小鼠耳蜗内,也不能同时观察到这些结构的全部,即内毛细胞带状突触的超微结构和形态可能表现出彼此之间存在明显区别的形式。结论在听力正常的生理状态下,成熟小鼠（c57BL/6J）耳蜗内毛细胞突触的形态和结构处于动态变化之中,突触的个体形态和结构可以表现出不同的特点,而这种结构上的动态变化可能和突触功能的变化有关。

英文摘要：

Objective To study the ultrastructue of afferent synapse of inner hair cells（IHCs） in C57BL/6J mice. Method The adult c57BL/6J mice with normal hearing function were selected,and the cochleas were taken out and the semi and ultra sections were performed for the ultrastructural observation by transmission electron microscope（TEM） . The ultrastructural properties within afferent synapses as well as structures nearby were analysized,and the potential correlation between the afferent synapses and other structures around was explored. Result The typical afferent synapse structure was observed including the presynaptic ribbon,synapse vesicles,the clear synapse cleft and the postsynaptic density（PSD） in mice with normal hearing ability. However,sometimes,those synaptic components could not be captured by TEM observation even in same cochlear section,but it did not mean the abnormilities of ribbon synaptic structures,and the muiltiple morphological shapes were shown in different normal ribbon synapse. Conclusion The ultrastructure of cochlear ribbon synapse in C57BL/6J mice is dynamic and the synaptic diversiform may correlate to the cochlear synaptic function in mice with normal hearing ability.