中文摘要：

The expression profile in the mouse hepatitis B virus X (HBx)-transfected model was investigated in order to lay a foundation for further study on the implication of cytokines expression in hepatitis B virus (HBV) infection.Hydrodynamic injection method via the tail vein was used to establish the animal HBx-transfected model.By using microassay,the differential expression of gene in each group was analyzed,which was further confirmed by using real-time PCR and semi-quantitative PCR.Most of chemokine genes such as Ccl2,Ccl5,Ccl9,MIG and IP-10 were up-regulated in the HBx-transfected mouse model versus the control mice,which was coincided with the microarray results.Western blotting and immunohistochemistry were applied to detect the expression of MIG and IP-10 in the liver tissues.Simultaneously,ELISA was adopted to measure the content of IFN-γ in the liver tissues.DNA microassay revealed that the expression of 611 genes changed in HBx-transfected mice as compared with that in pCMV-tag2B-transfected mice,and most of the screened chemokines were up-regulated (including MIG and IP-10).Additionally,IFN-γ protein levels were increased by 20.7% (P<0.05) in pCMV-tag2B-HBx-transfected mice as compared with the untreated mice.IFN-γ protein levels were reduced by 53.9% (P<0.05) in pCMV-tag2B-transfected mice as compared with the untreated mice,which was consistent with the up-regulation of MIG and IP-10.It was suggested HBx transfection could induce the expression of MIG and IP-10 in the liver tissues,which might play the roles in HBV-related liver immunity and cytokines-mediated antiviral effect.

英文摘要：

The expression profile in the mouse hepatitis B virus X （HBx）-transfected model was investigated in order to lay a foundation for further study on the implication of cytokines expression in hepa- titis B virus （HBV） infection. Hydrodynamic injection method via the tail vein was used to establish the animal HBx-transfected model. By using microassay, the differential expression of gene in each group was analyzed, which was further confirmed by using real-time PCR and semi-quantitative PCR. Most of chemokine genes such as Cc12, Cc15, Cc19, MIG and IP-10 were up-regulated in the HBx-transfected mouse model versus the control mice, which was coincided with the microarray results. Western blotting and immunohistochemistry were applied to detect the expression of MIG and IP-10 in the liver tissues. Simultaneously, ELISA was adopted to measure the content of IFN-y in the liver tissues. DNA mi- croassay revealed that the expression of 611 genes changed in HBx-transfected mice as compared with that in pCMV-tag2B-transfected mice, and most of the screened chemokines were up-regulated （includ- ing MIG and IP-10）. Additionally, IFN-y protein levels were increased by 20.7% （P〈0.05） in pCMV-tag2B-HBx-transfected mice as compared with the untreated mice. IFN-7 protein levels were reduced by 53.9% （P〈0.05） in pCMV-tag2B-transfected mice as compared with the untreated mice, which was consistent with the up-regulation of MIG and IP-10. It was suggested HBx transfection could induce the expression of MIG and IP-10 in the liver tissues, which might play the roles in HBV-related liver immunity and cytokines-mediated antiviral effect.