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中文摘要：
采用基于16SrDNA的变性凝胶梯度电泳（DGGE）技术研究了生活垃圾卫生填埋场和生物反应器填埋场覆土中Ⅱ型甲烷氧化菌群落结构,比较了不同填埋操作方式对Ⅱ型甲烷氧化菌的影响.结果表明,覆土铺有HDPE膜、无填埋气体渗入的填埋覆土中未发现Ⅱ型甲烷氧化菌;而有填埋气体渗入时,进行渗滤液亚表面灌溉的生物反应器填埋场,无论是否同时进行层内回灌,其覆土中均检测到甲烷孢囊菌（Methylocystis）.卫生填埋场填埋龄长达5a的填埋覆土中发现了甲基弯菌（Methylosinus）,填埋龄较低的填埋场覆土中未发现II型甲烷氧化菌.渗滤液亚表面灌溉及长时间填埋气体驯化能促进Ⅱ型甲烷氧化菌的生长.有机质等营养物质丰富而NH4＋-N浓度较低的填埋覆土有利于Ⅱ型甲烷氧化菌的生长.
英文摘要：
Adopting the denaturing gradient gel electrophoresis （DGGE） technique based on the 16S rDNA, the typeⅡ methanotroph community in the cover soils of sanitary landfill and bioreactor landfill was studied, the different landfill operation type on type Ⅱ methanotrophy influence was compared： In landfill cover soils with HDPE film, no landfill gas infiltration, no type Ⅱ methanotrophy was found; and when having landfill gas infiltrating, the bioreactor landfill sits of carrying out leachate subsurface irrigation, neglected it is on or not carrying out leachate recirculation in its cover soil all detected Methylocystis; in sanitary landfill sits, landfill age long reaching 5yeas landfill cover soil, Methylosinus was discovered; in landfill age shorten landfill cover soil did not discovered type Ⅱ methanotrophy. Leachate subsurface irrigation and long time landfill gas cultivation could promote type Ⅱ methanotrophy growth. The landfill cover soil of organics and nutrition rich and ammonia nitrogen concentration lower in landfill cover soil were favorable to type Ⅱ methanotrophy growth.
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