中文摘要：

目的：通过观察氧化苦参碱（oxymatrine,OMT）对神经病理性疼痛小鼠的脑、脊髓、背根神经结（DRG）的高电压依赖性钙通道（high voltage-dependent calcium channels,HVDCCs）辅助亚基mRNA表达量的影响,探讨氧化苦参碱的镇痛作用相关机制。方法：将15只C57BL/6小鼠随机分成假手术组、模型组、氧化苦参碱组,每组5只,通过部分结扎小鼠左右两侧后腿坐骨神经建立神经病理性疼痛模型,应用实时荧光定量PCR（real-time PCR）检测脑、脊髓、背根神经节（DRG）3个组织中高电压依赖性钙通道多种辅助亚基mRNA表达情况。结果：在脑组织中,模型组小鼠辅助亚基α2δ1、α2δ2的mRNA水平明显升高,α2δ3、α2δ4的mRNA水平明显降低。与模型组比较,氧化苦参碱组中α2δ1、α2δ2及γ1的mRNA水平明显著降低,α2δ4的mRNA水平明显升高。在脊髓组织中,模型组小鼠的α2δ1、α2δ2、α2δ3、γ1及γ4的mRNA水平均明显升高;氧化苦参碱（150mg/kg）处理使得其中模型组升高的α2δ1、α2δ2、α2δ3的mRNA水平显著降低。在DRG组织中,模型组小鼠α2δ1的mRNA水平明显升高,α2δ2、α2δ3的mRNA水平明显降低;氧化苦参碱（150mg/kg）处理使得其中模型组升高的α2δ1的mRNA水平明显降低。α2δ4、γ3在DRG组织中未见表达。各组织中,部分坐骨神经结扎及氧化苦参碱给药对其余β、γ等亚基的mRNA表达水平变化影响无统计学意义。结论：氧化苦参碱的镇痛作用与高电压依赖性钙通道辅助亚基α2δ1有高度相关性。

英文摘要：

Objective： To investigate the effects of oxymatrine on the mRNA expressions of high voltage-dependent calcium ehannelg auxiliary sub- units in brain, spinal cord and dorsal root ganglia tissues from neuropathic mice, and to further explore the analgesic mechanism of oxymatrine. Methods： Fifteen C57BL/6 mice were randomly divided into sham operation group, model group and oxymatrine group, and 5 in each group. Neuropathic pain models were established by partial sciatic nerve ligation on both the left and right hind legs of C57 BL/6 mice. Real-time quantitative PCR technique were used to detect the mRNA expressions of multiple auxiliary subunits for high voltage-dependent cal- cium channel in brain, spinal cord and dorsal root ganglia tissues from these mice. Results： In brain tissue, the mRNA expression levels of auxiliary subunit α2δ1 and α2δ2 were significantly increased, while those of α2δ3 and α2δ4 were significantly decreased in the model group mice （ P 〈 0.05 ）. Compared to this, in oxymatrine group, the mRNA levels of subunit α2δ1, α2δ2, 0α2δ3 and γ1 decreased significantly, but that of α2δ4 was increased significantly （ P 〈 0.05 ） ; In spinal cord tissue, the mRNA expressions of α2δ1, α2δ2, α2δ3, 71 and 3,4 were significantly increased in the model group mice（ P 〈 0.05）. These elevations of the mRNA level of et281, o282 and α2δ3 in the model group mice were attenuated by applying oxymatrine（150mg/kg） （ P 〈0.05） ; In DRG tissue, the mRNA level of α2δ1 was significantly in- creased and those of α2δ2 and α2δ3 were significantly decreased in the model group mice （ P 〈 0.05 ）, but administration of oxymatrine （150mg/kg） showed attenuation on the elevated mRNA level of α2δ1 significantly （P 〈 0.05）. And subunit α2δ4 and γ3 were not ex- pressed in DRG tissue. In all these tissues, both partial sciatic nerve ligation and oxymatrine administration didnt show any effects on the mRNA expressions of other β and γ subunits. Conclusi