中文摘要：

背景：体内骨组织工程是在骨或非骨部位植入具有骨诱导活性的生物材料,利用机体作为生物反应器去构建组织工程骨,以修复骨缺损。骨移植物所具备的优良生物相容性和机械性能为骨移植开辟新的治疗途径。然而,其相关干细胞来源尚不清楚。目的：探索骨髓间充质干细胞作为干细胞是否参与生物材料的异位诱导成骨。方法：分离纯化雄性beagle犬的骨髓间充质干细胞。将培养至第3代骨髓间充质干细胞移植入雌性beagle犬体内,建立同种异体骨髓移植模型,同期将双相钙磷陶瓷植入受体犬竖脊肌内。6周后,获取骨构建物标本,利用原位荧光杂交技术对骨移植物Y染色体进行示踪。结果与结论：原位荧光杂交结果显示Y染色体在骨构建物中有荧光信号表达,表明骨髓间充质干细胞可通过血液循环途径募集到异位生物材料处,形成骨组织。结果提示骨髓间充质干细胞参与生物材料异位诱导成骨,是生物材料异位诱导成骨干细胞来源之一。

英文摘要：

BACKGROUND：In vivo bone tissue engineering is to implant biological materials with bone induction activity in the bone or non-bone region.The body served as bioreactor to construct tissue engineered bone to repair bone defects.Bone transplant with good biocompatibility and mechanical function developed a new therapeutic pathway for bone transplantation.However,the source of related stem cells remains unclear.OBJECTIVE：To investigate whether bone marrow mesenchymal stem cells（BMSCs） participate in forming ectopic bone of biomaterial.METHODS：BMSCs were separated and purified from male beagle dogs.BMSCs at passage 3 were transplanted into the female beagle dogs to establish models of allogeneic bone marrow transplantation.Simultaneously,biphasic calcium phosphate ceramic was implanted in erector spinae of receptor dog.Following 6 weeks,bone sample was harvested.Fluorescence in situ hybridization was utilized to trace bone transplant Y chromosome.RESULTS AND CONCLUSION：Fluorescence in situ hybridization results demonstrated that chromosome Y had fluorescent signal expression in bone constructs,which suggested that BMSCs can be collected in the ectopic biomaterial region by blood circulation,and formed bone tissue.Results indicated that BMSCs participate in inducing ectopic bone formation,as a source of inducing ectopic bone formation.