对籼型两系骨干亲本9311(受体)和粳稻日本晴(供体)构建的单片段代换系群体,在芽期和苗期分别进行低温处理,以芽期处理后的恢复成活率和苗期卷叶程度作为耐冷性的评价指标进行耐冷性评价。结果表明,日本晴在芽期和苗期耐冷性极显著强于9311,不同代换系芽期和苗期耐冷性也存在极显著差异,芽期和苗期耐冷性较强的代换系X724、X732、X733较好地保持了9311的农艺性状,其产量等性状的一般配合力优于9311;鉴定出18个芽期耐冷性QTL和5个苗期耐冷性QTL,分布于12条染色体上的20个代换片段中,第12染色体的RM1261-RM519和RM17区域同时检测出芽期和苗期耐冷性QTL;芽期耐冷性QTLqCTP9、qCTP11.2、qCTP12.1和苗期耐冷性QTLqCTS1.1、qCTS1.2具有较大加性效应,对应代换系耐冷性较强。
The cold tolerance at plumule stage(CTP) and seedling stage(CTS) were respectively evaluated at 4℃ and 13℃/7℃(day/night) using a set of single segment substitution lines(SSSLs) derived from indica recipient(9311) and japonica donor(Nipponbare).The survival seedling rate and the normal leaf rate(NLR) were used as the estimation index for CTP and CTS,respectively.The results showed that the CTP and CTS of Nipponbare were extremely stronger than those of 9311,and the difference of CTP and CTS were extremely significant among SSSLs.The SSSLs(X724,X732 and X733) with strong CTP and CTS mostly kept the agronomic traits of 9311.The General Combing Ability(GCA) of these three SSSLs,such as yield GCA,were much better than that of 9311.A total of 18 quantitative trait loci(QTLs) for CTP,5 QTLs for CTS were identified among 20 introgression segments distributed on 12 chromosomes.Among these QTLs,the CTP QTLs(qCTP9,qCTP11.2 and qCTP12.1),and the CTS QTLs(qCTS1.1 and qCTS1.2) showed more additive effect,and the corresponding SSSLs of those QTLs displayed stronger CTP and CTS,respectively.