中文摘要：

目的探究锂盐对大鼠正畸牙保持阶段牙周膜中成骨标记物Runx2和Osterix表达量的影响。方法 42只Wistar大鼠,建立正畸牙保持模型,牙移动后保持第0天时处死6只动物作为基线,其余随机分为实验组和对照组,分别给予同剂量的Li Cl和生理盐水灌胃处理,于保持后的第3、7、14天处死,取上颌骨磨牙段经HE染色及Runx2免和Osterix免疫组织化学染色,研究牙移动后保持阶段第一磨牙远中根的远中面牙周组织的结构变化以及Runx2免和Osterix的表达。结果随着保持时间的增加,实验组与对照组研究区域均出现成骨现象,Runx2免和Osterix的表达均增高,Li Cl组成骨现象更为活跃,在第7天和第14天时,Li Cl组Runx2的表达量显著性高于对照组,第14天时,Li Cl组Osterix的表达量显著性高于对照组。结论 Li Cl可以促进正畸牙保持阶段牙周组织中Runx2和Osterix表达量的增加,促进成骨。

英文摘要：

Objective To study the change of expression of Runx2 and Osterix induced by Lithium during orthodontic retention period in periodontium. Methods Wistar rats were subjected to put orthodontic orthodontic tooth movement for 2 weeks. Six rats were sacrificed immediately as initial control, the rest of rats were randomly divided into Li Cl group received garage-fed daily with Li Cl and control group with saline of the same dose.Samples were collected at3,7 and 14 day during retention period. The results were assessed by hematoxylin and eosin staining（HE） and immunohistochemisrty of Runx2 and Osterix. Results During retention period, the osteogenic activity of Li Cl group was significantly more active than the controls. There was statistical significant difference between Li Cl group and control group in expression of Runx2 on 7day and 14 day while in expression of Osterix on 14 day. Conclusion Lithium could promote osteogenic activity by increase of expression of Runx2 and Osterix during orthodontic retention stage.