调查内乳开发的基因表示侧面,一个 cDNA 图书馆在不同发育阶段从椰子的肉被构造并且描绘。构造 cDNA 图书馆合并了 107 总共克隆的约 1 ×,并且插入碎片的尺寸从 800 ~ 2 000 bp 。定序 100 随机拣的克隆的结果证明再结合率是 96% 。在随后的顺序分析, 41 克隆(41%) 对已知的功能蛋白质相应,并且 23 克隆与不同工厂的相应基因显示出高氨基酸身份(超过 80%) 。半量的 RT-PCR 显示人造奶油在和血球素基因是肉特定的表示,并且在不同发育阶段有微分表示水平。克隆 29,是认出了对 KIAA1239 蛋白质相应(人现代人) ,被观察发生九次,显示这基因可能在内乳发展阶段期间是过去表示的。然而,相应蛋白质仅仅在哺乳动物被发现,并且详细功能仍然是未知的。这些基因的功能的描述的说明将立即被执行。
To investigate the gene expression profile of endosperm development, a cDNA library was constructed and characterized from the pulp of coconut at different developmental stages. The constructed cDNA library incorporated approximately 1 × 10^7 clones in total, and the size of the insertion fragments ranged from 800 to 2 000 bp. Sequencing results of 100 randomly picked clones showed that the recombination rate was 96%. In subsequent sequence analysis, 41 clones (41%) were homologous to known function proteins, and 23 clones showed high amino acid identity (more than 80%) with the corresponding genes of different plants. Semi-quantitative RT-PCR indicated that oleosin and globulin genes are pulpspecific expression, and have differential expression level in different developmental stage. Clone 29, recognized as homologous to KIAA1239 protein (Homo sapiens), was observed to occur nine times, indicating that this gene may be over-expressed during the endosperm development stage. However, the homologous protein was found only in mammals, and the detailed function is still unknown. Elucidation of the functional characterization of these genes will be carried out immediately.