中文摘要：

目的：观察原儿茶醛对ox-LDL诱导的人脐静脉血管内皮细胞株（CRL-1730）损伤的保护作用。方法：ox-LDL诱导人血管内皮细胞损伤，并以不同剂量的原儿茶醛干预24h，用四甲基偶氮唑蓝比色法检测细胞存活数量，比色法测定细胞培养液中NO含量和NOS活性，流式细胞术测定细胞内CD40蛋白表达。结果：原儿茶醛对ox-LDL引起的CRL-1730损伤血管内皮细胞数量的减少和培养液中NO、NOS的降低有明显的抑制作用；ox-LDL可引起细胞内CIM0蛋白表达增加，原儿茶醛可以抑制此作用。结论：原儿茶醛对ox-LDL诱导的血管内皮细胞损伤具有保护作用，其机制可能与CD40／CD40L抗炎途径有关。

英文摘要：

Objective： To observe the protective effects of protocatechualdehyde on the human umbilical vein endothelial cells （CRL-1730） induced injury by ox-LDL. Methods： The CRL-1730 were induced injury by ox-LDL, and then treated with protocatechu- aldehydes for 24 hours. The cytoactive of CRL-1730 was assessed by colorimetry of MTT, the NO level and NOS activity in the cell cul- ture fluid were observed by colorimetry, and the expression of CD40 protein was determined by flow cytometry. Results ： Compared with the ox-LDL group, protocatechualdehyde increased the number of CRL-1730 and the level of NO and NOS in cell culture fluid. Be- sides, protocatechualdehyde decreased the expression of CD40 protein, which was increased by ox-LDL. Conclusion ： Protocatechualde- hyde has protective effect on the CRL-1730 induced injury by ox-LDL and its mechanism of action may be related to the CD40/CD40L pathway.