中文摘要：

目的进一步探讨缺氧后血管新生的调控机制。方法常规方法培养人微血管内皮细胞,低氧培养箱制备内皮细胞缺氧模型（观察组）,正常细胞作为对照组。采用real time PCR法检测两组内皮特异性microRNA（miR-210、miR-92a、miR-126）表达变化。结果与对照组比较,miR-210、miR-92a、miR-126分别上调了（5.19±0.99）、（3.02±0.88）、（3.87±0.83）倍,P均〈0.05。结论缺氧可促使内皮细胞特异性microRNA表达改变,此可能为缺氧后血管新生的主要调控机制。

英文摘要：

Objective To investigate the control mechanism of angiogenesis after hypoxia.Methods Human microcapillary endothelial cells（HMEC）were cultured commonly,and hypoxia model of endothelial cells was prepared with hypoxia incubator（observed group）,the normal cells was taken as control group.The expression of miR-210,miR-92a,miR-126 were detected by real time PCR method.Results It showed a significant up-regulation of miR-210,miR-92a,miR-126 in observed group compared to control group,about 5.19±0.99,3.02±0.88 and 3.87±0.83 fold changes respectively.Conclusion Hypoxia can promote the changes of specific microRNA in endothelial cells,which maybe the main control mechanism of angiogenesis after hypoxia.