中文摘要：

目的探讨Cullin1（Cul1）基因对乳腺癌细胞血管形成的影响及机制。方法使用阴性对照慢病毒（shCtr1）和Cull干涉慢病毒（shCul1）分别转染MDA—MB-231和BT-549细胞，并分别标记为231shCtr1组、231shCul1组和549shCtr1组、549shCul1组。体外血管生成实验观察2种乳腺癌细胞中Cul1干涉后血管生成能力的变化。Westernblot法检测Cul1、血管内皮生长因子（VEGF）、缺氧诱导因子-1α（HIF-1α）的表达。结果与shCtr1组相比，MDA-MB-231和BT-549两种细胞shCul1组形成的毛细血管数目分别减少了96．90％和95．14％；Cul1蛋白表达量分别减少了93．48％和94．85％；VEGF蛋白表达量分别减少了87．18％和93．88％；HIF-1α蛋白表达量分别减少了88．05％和92．25％。结论在乳腺癌细胞中干涉Cul1基因能够下调VEGF和HIF-1α的表达并抑制乳腺癌细胞血管生成。

英文摘要：

Objective To investigate the role of Cul1in1 （ Cul1 ） gene in the angiogenesisof human breast cancer cell. MethodsThe negative control lentivirus（ shCtrl ） and Cul1 interfering lentivirus （shCul1） weretransfected into MDA -MB-231 and BT- 549 breast cancer celllines which were separateiynamed after 231 -shCtrl group, 231 -shCul1 group, 549 - shCtrl group and 549 - shCul1 group. The angiogenesis ability of two breast cancer cell lines with Cul1 in- terfered were examined by in vitro angiogenesis experiment. The expressions of Cul1, vascular endothelial growth factor （VEGF）, hypoxia inducible factor-1α（HIF- 1α） were detected by using Western blot test in both breast cancer cells after silencing Cul1. Results Compared with the shCtrl groups, the numberof capillaries were decreased by 96.90% and 95.14% in231 -shCul1 group and 549 -shCul1group. The Cul1 protein level were significantly decreased by 93. 48% and 94.85% , the VEGF expression were suppressed by 87.18% and 93.88% and the HIF - 1α expression were decreased by 88.05% and 92.25% in 231 - shCul1 group and 549 - shCul1 group after Cul1 interfered. Conclusion Si- lencing Cul1 could downregulate VEGF and HIF - 1α expression to inhibit the angiogenesis ability in human breast canc- er.