中文摘要：

目的评价静电纺丝法制备的PLGA/可溶性蛋壳膜蛋白（soluble eggshell membrane protein,SEP）纤维膜的理化性能和生物相容性。方法利用静电纺丝技术制备PLGA∶SEP=90∶10、70∶30、50∶50的PLGA/SEP纳米纤维膜（实验组）及PLGA纤维膜（对照组）。扫描电镜观察其表面形貌,测量纤维直径。检测接触角、拉伸强度。傅里叶红外光谱分析混合物构象,扫描电镜和MTT法研究成纤维细胞在纤维膜表面生长、增殖情况,评价PLGA/SEP纤维膜的细胞相容性。结果两组纤维膜直径均一,呈相互连通的多孔网状结构。随着PLGA含量的增加,纤维直径、接触角、拉伸强度增大;红外光谱分析表明PLGA与SEP的特征峰均出现在复合膜的红外谱图中;SEM观察PLGA/SEP纤维膜组细胞粘附数目较PLGA纤维膜组多,生长旺盛,并且已有细胞向纤维内部生长;MTT结果也证明成纤维细胞在PLGA/SEP纤维膜上增殖情况优于PLGA纤维膜。结论电纺制备的PLGA/SEP纤维膜既有良好生物相容性又具备一定力学强度,可作为一种具有发展潜力的引导组织再生（guided tissueregeneration,GTR）膜。

英文摘要：

Objective To evaluate the physicochemical properties and biocompatibility of PLGA/SEP（soluble eggshell membrane protein） nanofiber membrane prepared by electrospinning.Methods Different ratios of electrospun PLGA/SEP nanofiber membrane（experimental group） and pure PLGA membrane（control group） were prepared.The ratios of PLGA/SEP were 90∶10,70∶30,50∶50.The blend membrane and its fiber diameter were abserved with scanning electron microscope（SEM）.The contact angle and tensile strength were tested.The constitution of the blend membrane were analyzed with Fourier infrared spectroscopy.The growth and proliferation of fiberous in fiberous membrane were examined by MTT to determine the biocompatibility of PLGA/SEP nanofiber membrane.Results Uniform diameter and interconnected porous network structure showed in nanofiber membrane（including in experimental and control group）.Fiber diameter,contact angle and tensile strength were increased with PLGA added.There were characteristic peaks of PLGA and SEP in the infrared spectra of blend membrane.Fibroblast growth in the PLGA/SEP fiber membrane was better than that in pure PLGA fiber membrane.Conclusions There is good biocompatibility and adapt tensile strength in the electrospun PLGA/SEP mat.It is a potential material for guided tissue regeneration membrane.