中文摘要：

目的：建立以胶柬电动毛细管色谱（MECC）法同时测定足光散中苯甲酸、水杨酸含量的方法。方法：采用未涂层石英毛细管（75μm×55cm，有效长度47cm），以20mmol·L^-1硼砂-30mmol·L^-1十二烷基硫酸钠-4％甲醇（pH9．8）为运行缓冲液，以抗坏血酸为内标，检测波长为250nm。结果：苯甲酸和水杨酸的检测浓度分别在120～320／Lg·mL^-1（r=0．9998）和80-320μg·mL^-1（r=0．9995）范围内与各自对内标峰面积的比值呈良好线性关系；平均回收率分别为98．37％和100．69％，RSD分别为4．47％和1．61％（n均=9）。结论：本方法简单、灵敏、快速、回收率高、重现性好，可用于足光散的质量控制。

英文摘要：

OBJECTIVE： A micellar electrokinetic capillary chromatography（MECC） method was established for determination of benzoic acid and salicylic acid in Zuguang san. METHODS： An uncoated silica capillary（75 μm × 55 cm, effective length 47 cm） was used with 20 mmol · L^- 1 borate - 30 mmol · L^- 1 SDS- 4% methanol（pH 9.8） as the running buffer solution. The internal standard was antiscorbic acid. The detection wavelength was set at 250 nm. RESULTS： The calibration curves were linear in the range of 120--320 μg 12· mL^- 1 for benzoic acid（ r = 0.999 8） and 80--320 μg · mL^- 1（ r = 0.999 5） for salicylic acid. The average recoveries were 98.37% and 100.69%, respectively and the RSD were 4.47% （n = 9） and 1.61% （n = 9）,respectively. CONCLUSION： MECC method was proved to be simple, sensitive and reproducible with high recovery rate and it is applicable for the determination of the contents of benzoic acid and salicylic acid in Zuguang san.