中文摘要：

为探讨外源性二氧化硫（sulfur dioxide,SO2）对脂多糖（lipopolysaccharide,LPS）所致大鼠肺损伤时肺组织炎性反应的影响,采用经尾静脉注射LPS的方法复制大鼠肺损伤模型,将雄性Sprague-Dawley（SD）大鼠随机分为4组：对照组、LPS组、LPS＋SO2组[注射LPS前10min静脉给予外源性SO2供体亚硫酸钠/亚硫酸氢钠（Na2SO3/NaHSO3）0.5mL]和SO2组（单纯给予Na2SO3/NaHSO3）。给药后6h处死大鼠,测定肺系数;光镜下观察肺组织形态学改变;测定支气管肺泡灌洗液（bronchoalveolar lavage fluid,BALF）中的中性粒细胞（polymorphonuclear neutrophil,PMN）数量;采用流式细胞术检测肺组织中细胞间黏附因子-1（ICAM-1）的表达情况;应用酶联免疫法（ELISA）检测血清中IL-1、IL-6和IL-10含量。所得结果如下：与对照组比较,静脉注射LPS可引起肺组织明显的形态学改变;肺系数、肺损伤的组织学定量评价指标（IQA）和BALF中PMN数量增加;肺组织中ICAM-1表达增多,血清中IL-1、IL-6和IL-10水平升高。预先给予Na2SO3/NaHSO3可显著减轻LPS所致肺损伤的程度,同时使肺系数、肺IQA和BALF中PMN数量减少;肺组织中ICAM-1表达下降,血清中IL-1、IL-6水平下降而IL-10水平升高。SO2组大鼠上述各项指标与对照组大鼠相比无显著差异。以上结果提示,外源性SO2可抑制LPS所致大鼠肺损伤时肺组织中的炎症反应。

英文摘要：

To investigate the influence of sulfur dioxide （SO2） on lipopolysaccharide （LPS）-induced acute lung injury （ALI）, we examined the influence of exogenous SO2 on pulmonary tissue inflammatory response. A rat model of ALl induced by intravenous （IV） injection of LPS was developed. Male Sprague-Dawley （SD） rats were divided into four groups randomly： control group, LPS group, LPS plus SO2 group （IV injection of 0.5 mL Na2SO3/NaHSO3 10 min before LPS administration） and SO2 group （only given NaESO3/NaHSO3）. Animals were sacrificed 6 h after agent administration. Lung weight/body weight ratio （LW/BW） was measured and calculated. Morphological changes of lung tissues were observed. The number of polymorphonuclear neutrophil （PMN） in the bronchoalveolar lavage fluid （BALF）, intercellular adhesion factor-1 （ICAM-1） expression in the lung tissue and IL-1, IL-6 and IL-10 levels in the serum were tested. The results showed that, compared to control rats, the LPS-treated rats had severe injuries of lung tissues and an increased LW/BW, increased index of quantitative assessment （IQA） score, increased PMN number in the BALF, increased ICAM-1 expression in the lung tissue and increased IL-1, IL-6 and IL-10 levels in the serum 6 h after LPS injection. Administration of the SO2 donor, Na2SO3/NaHSO3, into LPS-treated rats reduced the LW/BW, PMN number and ICAM-1 expression, and alleviated the degree of ALI （measured by the IQA score）. In addition, Na2SO3/NaHSO3 decreased IL-1 and IL-6 levels, but increased IL-10 level in the serum. There were no significant differences in the above indexes between SO2-treated rats and control rats. These results suggest that exogenous SO2 could inhibit the pulmonary tissue inflammatory response in rats with LPS-induced ALI.