中文摘要：

探索利用蛋白质组学的方法筛选胰腺干细胞的标记物．通过大鼠胰腺大部分切除（Px）的方法建立胰腺增生模型，采用双向电泳（2DE）分离比较Px术后第3天大鼠增生胰腺组织与假手术（Sx）非增生胰腺组织蛋白质的表达谱差异，用基质辅助激光解吸离子化飞行时间质谱（MALDI-TOF-MS）和肽质量指纹图谱（PMF）的方法对差异蛋白进行鉴定，进一步经生物信息学检索，筛选与胚胎发育和细胞分化相关的蛋白质分子。蛋白质印迹分析蛋白质HSP47、Vimentin在Px大鼠胰腺组织中的表达，以验证2DE的结果．2DE显示Sx和Px大鼠胰腺组织平均蛋白质点数分别为（1369±31）和（1315±28）个，其中共有91个蛋白质点出现1．5倍以上的表达差异．所有差异蛋白质点PMF鉴定出53种蛋白质，其中Vimentin，cytokeratin 8（CK8），lymphocyte cytosolic protein 1（L-plastin），heterogeneous nuclear ribonucleoprotein A2／B1（hnRNP A2／B1）和L-arginine：glycine amidinotransferase（AGAT）与胚胎发育和细胞分化有关，这些蛋白质可能是胰腺干细胞潜在的候选标记物．总之，蛋白质组学的方法利用其高通量的特点能有效发现潜在的胰腺干细胞生物学标记物．

英文摘要：

In order to examine potential molecular markers of pancreatic stem cells, a regeneration pancreatic model was induced by 90% partial pancreatectomy （Px） in rats. Changes in the protein expression in rat regeneration pancreas at 3rd day after Px, as comparing to sham surgery （Sx）, were analyzed by using two-dimensional gel electrophoresis （2DE）, mass spectrometry （MS）, and peptide mass fingerprint （PMF） based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and database searching. HSP47 and Vimentin were analyzed in Px and Sx rat with Western blotting to confirm the results of 2DE. The average spots in gels for Sx and Px pancreatic tissues were （1 369 ± 31） and （1 315 ± 28）, respectively. 2DE displayed 91 spots with at least 1.5-fold of differential expression at the time point of 3 days after pancreatectomy and 53 differentially expressed proteins were identified by PMF. Among them, Vimentin, CK8, L-plastin, hnRNP A2/B 1 and AGAT are associated with embryogenesis and cells differentiation and may be new potential pancreatic stem cells markers. The proteome profiling technique provided a broad-based and effective approach for discovering potential biomarkers of pancreatic stem cells.