中文摘要：

目的分析泛耐药肺炎克雷伯菌JM45株携带的喹诺酮类药物耐药基因，研究其对喹诺酮类药物的耐药机制。方法采用Roche454高通量测序技术对肺炎克雷伯菌JM45株做全基因组测序（完成图），分析喹诺酮类耐药基因携带状况，再将gyrA与pare全长基因与其他6株肺炎克雷伯菌做分子进化分析。结果最终得到JM45株一条完整的基因组（染色体）序列及两条质粒序列；基因组（染色体）序列大小为5273812bp（GC含量65．8％），质粒1序列大小为317156bp（GC含量53．0％），质粒2序列大小为12209bp（GC含量55．3％）；在基因组（染色体）序列中携带了gyrA基因（全基因组FeatureID：KPN-1614），parC基因（FeatureID：KPN-0743）；与肺炎克雷伯菌喹诺酮类药物敏感株相比较，gyrA基因第83位密码子由TCC→ATC，翻译成氨基酸序列后丝氨酸（Ser）→异亮氨酸（Ile），parC基因第80位密码子由AGC→ATC，翻译成氨基酸序列后丝氨酸（Ser）→异亮氨酸（Ile）。结论肺炎克雷伯菌JM45株喹诺酮类药物耐药是gyrA基因和parC基因喹诺酮耐药决定区（QRDR）突变所致；gyrA与parC全长基因的分子进化分析提示JM45株与肺炎克雷伯菌HSll286关系最为接近（序列相同），与肺炎克雷伯菌342关系最远。

英文摘要：

OBJECTIVE To investigate the quinolone-resistance genes in pandrug-resistant Klebsiella pneumoniae JM45 and study the drug resistance mechanisms of the strain to the quinolones antibiotics. METHODS The whole genome sequencing （ completed graph）was performed by using high throughput Roche 454 sequencing approach, then the quinolone-resistance genes were analyzed, and the molecular evolutionary analysis was performed with full-length gyrA and parC between JM45 and other 6 K. pneumoniae isolates. RESULTS A complete gerlome （chromosome） sequence and 2 plasmids sequences were obtained in JM45. The size of chromosome was 5 273 812 bp （GC content： 65.8%）, the size of plasmid 1 was 317 156 bp （GC content.. 53.0%）, and the size of plasmid 2 was 12 209 bp （ GC content ： 55.3 % ）. gyrA （ Feature ID.. KPN_ 1614） and parC （ Feature ID： KPN_0743） were positive in chromosome. As compared with the K. pneumoniae strains which were sensitive to quinolor~es, the 83 rd codon of gyrA in JM45 changed from TCC to ATC（Ser-~Ile）, and the 80th codon of parC in JM45 changed from AGC to ATC（Ser--~Ile）. CONCLUSION Mutations of gyrA and parC in quinolone resist- anee-determining region （QRDR） play a key role in quinolone resistance of JM45. And the moIecular evolutionary analysis of full-length gyrA and parC suggests that the closest relationship exists between JM45 and K. pneumon- iae subsp, pneumoniae HSl1286 （the same sequence）, and that the farthest relationship exists between JM45 and K. pneumoniae 3 4 2.