中文摘要：

利用8对已发表的翘嘴鳜微卫星引物,对5种鳜属鱼类翘嘴鳜、大眼鳜、斑鳜、波纹鳜和暗鳜共147个个体进行跨种扩增和遗传多样性的研究。结果发现,8个微卫星标记除位点HW8在波纹鳜中无多态性外,其他位点在5种鳜属鱼类中都具有较高的多态性。每个位点等位基因数为4~8个不等,平均等位基因数为6.13;在5个物种中分析的平均有效等位基因数为3.58,平均观测和期望杂合度分别为0.75和0.59,平均多态性信息含量0.51,为高度多态,表明鳜属鱼类物种的遗传多样性比较丰富。采用Nei’s遗传距离和遗传相似性系数分析5种鳜属鱼类系统发育关系,并构建UPGMA聚类图,结果显示翘嘴鳜和大眼鳜、斑鳜和波纹鳜分别有较近的亲缘关系,斑鳜和暗鳜亲缘关系最远。

英文摘要：

By using eight microsatellite primer pairs,cross-species amplification and genetic diversity analysis were carried out from a total of 147 individuals among five species of Siniperca,namely S.chuatsi,S.kneri,S.scherzeri,S.undulata and S.obscura.The results showed that except for loci HW8 of S.undulate,eight microsatellite loci in the five species of Siniperca were highly polymorphic.The numbers of alleles per locus ranged from 4to 8,and the mean allele numbers was 6.13.The average effective number of alleles of the five species was 3.58,with the average observed and expected heterozygosity being 0.75 and 0.59,respectively.The average polymorphism information content was 0.51.All these data showed that the eight microsatellite markers had high sensibility,and could be used for species identification and analysis in the sinipercine fishes.Using Nei＇s genetic distance and genetic similarity coefficient,the UPGMA clustering showed that the genetic relationship between S.chuatsi and S.kneri,S.scherzeri and S.undulata were relatively closer,while genetic relationship between S.scherzeri and S.obscura was the furthest.