中文摘要：

目的探讨小分子干扰RNA（siRNA）靶向沉默胶质瘤细胞系U87中组织蛋白酶D（CD）对胶质瘤细胞生物学行为的影响。方法采用脂质体介导的CDsiRNA、scramblesiRNA、空白对照siRNA转染体外常规培养的胶质瘤细胞株U87，RT．PCR、Westernblotting分别检测转染后U87细胞CDmRNA和蛋白的表达；MTT法检测转染CDsiRNA、scramblesiRNA1～5d后U87细胞的增殖；细胞侵袭实验检测转染CDsiRNA、scramblesiRNA48h后细胞侵袭能力的变化。结果转染48h后CDsiRNA组细胞CDmRNA、蛋白的表达明显低于转染scramblesiRNA和空白对照siRNA组，差异有统计学意义（P〈0．05）；转染后2、3、4、5d转染CDsiRNA组U87细胞吸光度似）值低于转染scramblesiRNA组，差异有统计学意义（P〈0．05）；细胞侵袭实验结果表明转染CDsiRNA组滤膜细胞数低于转染scramblesiRNA组，差异有统计学意义（氏0．05）。结论CD可能是具有广泛应用前景的胶质瘤分子生物治疗的靶点。

英文摘要：

Objective To explore the effect of small interfering RNA （siRNA）-mediated cathepsin D （CD） knock-down on biological behavior of U87 cells. Methods CD-siRNA, scramble siRNA and control siRNA mediated by lipofectamin were introduced into the U87 cells, respectively. The silencing effect of CD was verified by RT-PCR and Western blotting in terms of CD mRNA and protein expressions. The cell viability of scramble and CD siRNA treated U87 cells was measured by MTT on 1-5 d after transfection. The invasive potential of scramble and CD siRNA treated U87 cells was measured by Transwell coated with Matrigel 48 h after trausfection. Results Forty-eight h after transfection, mRNA and protein expressions of CD in CD siRNA treated U87 cells were remarkably reduced as compared with those in the scramble siRNA and control siRNA treated U87 cells （P〈0.05）. The absorbance （A value） of CD siRNA treated U87 cells was significantly lower than that in the scramble siRNA treated U87 cells 2, 3, 4 and 5 d after transfection （P〈0.05）; and the invasive potential of CD siRNA treated U87 cells was suppressed significantly as compared with that in the scramble siRNA treated U87 cells （P〈0.05）. Conclusion CD has the potential to be a promising therapeutic target.