中文摘要：

目的探讨精液凝固蛋白（踺）在肾癌患者癌旁组织及癌组织中的表达及临床意义。方法收集具有明确病理诊断的20例肾癌组织及癌旁组织，应用qRT-PCR和Westernblot方法检测＆基因和蛋白水平的表达，并进一步研究了％与临床分期之间的相关性。结果肾癌组织标本中SgmRNA的相对表达量为（O．71±0．48），明显低于癌旁组织中的（1．07±0．44）（P〈0．05）。在Ⅲ／Ⅳ期癌组织中SgmRNA的表达量为（O40±0．19），明显低于I／Ⅱ期癌组织的（0．92±0．51）（P〈O．05）；Sg蛋白表达亦表现出相同趋势。结论Sg基因在肾癌中的异常表达与肾癌的发生发展关系密切。

英文摘要：

Objective To investigate the expression and clinical significance of semenogelin（Sg） gene in renal cell carcinoma（RCC）. Methods The expressions of Sg were detected by quantitative- PCR （qRT-PCR） and Western blot in 20 RCC samples and tumor-adjacent tissues, respectively. Results The expression of Sg mRNA in RCC tissues was 0. 71±0. 48,which was lower than 1.07±0.44 in tumor-adjacent tissues（P〈0. 05）. The expression of Sg mRNA in stage Ⅲ/Ⅳ RCC tissues was lower than that in stage I-Ⅱ（0. 40±0. 19 vs. 0. 92±0. 51） （P〈0. 05）. The expresion of Sg protein displayed the same tendency. Conclusion The abnormal expression of Sg in RCC may be closely related to the development and peogress of renal cell carcinoma.