中文摘要：

目的：观察速效救心丸和通心络胶囊对大鼠药物代谢酶细胞色素P450（CYP450）的影响。方法：给予大鼠试验药物后制备肝及小肠微粒体,CO还原差示光谱法测定肝微粒体CYP450含量,蛋白免疫印迹法检测肝微粒体中CYP1A2、CYP2C11、CYP2E1、CYP3A和肠微粒体中CYP3A蛋白的表达量。结果：速效救心丸组和通心络胶囊组与空白对照组比较,大鼠肝脏CYP450含量无显著变化（P〉0.05）;但是West-ern blotting实验对肝脏和肠道中多种CYP450酶表达量测定的结果显示,速效救心丸可诱导肝脏CYP1A2表达,但抑制肝脏CYP2C11和肠道CYP3A表达（P〈0.05）;通心络胶囊可诱导大鼠肝脏CYP1A2、CYP2E1蛋白表达（P〈0.05）。结论：速效救心丸和通心络胶囊对大鼠肝脏CYP450酶总量无影响,但是,在蛋白质水平对特定亚型蛋白的表达量有影响,由此可能引发的药物相互作用不容忽视。

英文摘要：

AIM：To investigate the influence of Quick-Acting Heart Reliever and Tongxinluo capsules on drug metablic enzyme cytochrome P450（CYP450） system in rats. METHODS： Rat liver and intestine microsome were prepared after a ten-day continuous administration of Quick-Acting Heart Reliever and Tongxinluo capsules.The content of total CYP450s in rat liver microsome was measured by UV-visible absorption spectrometry.Meanwhile,the protein expression of CYP1A2,CYP2C11,CYP2E1 and CYP3A in the liver microsome and CYP3A in the small intestine microsome was detected by western blotting assay.RESULTS：No significant difference of total CYP450 content was observed between each treatment group and control group（P0.05）.Western blot analysis showed that Quick-Acting Heart Reliever induced the CYP1A2 in liver microsome,but inhibited the liver CYP2C11 and CYP3A in intestine microsome;Tongxinluo capsules induced the expression of CYP1A2 and CYP2E1 in liver microsome.CONCLUSION： Quick-Acting Heart Reliever and Tongxinluo capsules exhibit induction/inhibition on the expression of CYP450 isoforms.The results suggest that the related possible side effects of drug-drug interaction could not be neglected in the period of clinical therapy.