中文摘要：

过度放牧导致高寒草地生态系统退化，围封是生态保护和恢复的管理手段。以青藏高原那曲县古露镇过牧退化高寒草地为对象，系统分析了高寒草地生态系统的植被特征及土壤理化特性、土壤酶活性、土壤微生物生物量和群落结构对围封的响应。结果表明，短期围封后，（1）植被平均高度、盖度和地上生物量均有极显著增加（P〈0.01），而生物多样性指数则显著降低（P〈0.01）；（2）土壤的水溶性有机碳含量、土壤物理结构（沙土与粉土的比例）及pH有显著变化（P 〈0.05）；（3）土壤酶活性没有明显改善；（4）土壤微生物生物量（细菌、放线菌、真菌）均呈显著增加（P 〈0.05）；（5）土壤中细菌的多样性有增加的趋势，其群落组成在门水平上也发生了变化；（6）Mantel test分析显示与土壤细菌群落结构的呈正相关性的环境因子主要为土壤有机碳含量（TOC）、总氮含量（TN）、碳磷比（C/P）与氮磷比（N/P）（P 〈0.05）。这表明围栏封育有利于藏北草地植被、土壤理化特性的恢复，还能维持土壤微生物多样性，促进高寒草地生态系统的可持续发展。

英文摘要：

Alpine grasslands, an important terrestrial ecosystem, play an essential role in global carbon and nitrogen cycles. Recently, overgrazing in alpine grasslands, particularly on the Tibetan Plateau, a sensitive district subject to climate change, is a serious issue leading to their degradation and degeneration, Fencing to exclude grazers has become an important management practice to protect and re-establish the degraded grasslands on the TP. Previous studies on the alpine grassland ecosystem have focused on a certain aspect. However, a systematic study has largely been lacking. The current research was focused on the severely degraded alpine grassland in Gulu Town, Naqu County, located in the north of TP. In order to determine the effect of restoration, we studied the short-term （three-year） effects of fencing on the vegetation characteristics, soil physicochemical properties, soil enzyme activities, soil microbial biomass, and soil microbial communities, and compared them with areas where grazing was allowed. Soil enzyme activity was detected using an UV spectrophotometer. Microbial biomass was measured by phospholipid-derived fatty-acid analysis （PLFA） method. Furthermore, we also studied profiling of complex soil bacterial communities based on 16S ribosomal RNA gene （rDNA） sequences by means of Next Generation Sequencing （NGS） technologies at the Ion Personal Genome Machine （PGM） platform. After fencing for three years, the alpine grassland ecosystem differed noticeably between the fencing and grazing areas across the study sites. Our study showed that： （1） The total plant cover, average vegetation height, and aboveground biomass of vegetation determined during the above ground vegetation surveys were significantly higher in fenced areas than the areas where grazing was permitted （P〈0.01）. However, the diversity of vegetation （Shannon-Weiner index） in the fenced area was significantly lower than that in grazing sites （P〈0.01）. （2） Soil pH perceptibly decreased while