中文摘要：

【目的】分析柑橘木虱（Diaphorinacitri）体内可能与黄龙病菌（CandidatusLiberibacter asiaticus）互作的内生细菌，为黄龙病菌的人工培养及其病害防控奠定基础。【方法】首先通过传统分离培养方法比较不同地理来源带黄龙病菌（带菌）和不带菌黄龙病菌（不带菌）的木虱中可培养内生细菌的差异。其次将带菌状况不同的木虱分别分为头、胸、腹3部分，经PCR扩增其16S rDNA的V6-V8区，用变性梯度凝胶电泳（PCR-DGGE）方法，比较带菌状况不同的木虱内生细菌的差异和木虱不同部位内生细菌的差异。选择3种差异的内生细菌：Bacillus sp.、Salmonella sp.、Enterobacter sp.，在8份带菌状况不同的木虱样品中通过q-PCR分别对其进行实时荧光定量分析，再以总细菌量为校正计算包括黄龙病菌在内的4种细菌的相对含量，数据经LSD检验，以各种细菌相对含量的-lg值作图，先比较同一样品中3种细菌分别与黄龙病菌的相对含量关系，再比较同种细菌在不同样品中的特性，分析3种内生细菌和黄龙病菌的互作关系。【结果】不带菌木虱中可培养内生菌菌落丰富度和菌落形成单位均大于带菌木虱中。在不带菌木虱中共获得14株形态不同的菌株，分属于芽孢杆菌属（Bacillus，3株）、欧文氏菌属（Erwinia，1株）、克雷伯氏杆菌属（Klebsiella，1株）、葡萄球菌属（Staphylococcus，2株）、节杆菌属（Arthrobacter，1株）、泛菌属（Pantoea，2株）、果胶杆菌属（Pectobacterium，1株）、沙门氏菌属等（Salmonella，1株）、链霉菌属（Streptomyces，1株）、Massilia brevitalea（1株）等10个细菌属。在带菌木虱中分得的4株细菌在不带菌木虱中均分离到，分属于克雷伯氏菌属、芽孢杆菌属、果胶杆菌属。其中Dc-11（嗜气芽孢杆菌属）在带菌木虱和不带菌木虱中分离频率均达到100%，表明其为木虱体内常驻细菌；?

英文摘要：

[Objective]The objective of this study is to analyze the endophytic bacteria in Diaphorina citri which may affect the pathogenesis of Candidatus Liberibacter asiaticus, and to provide a basis for artificial culture of Ca. L. asiaticus, prevent and control of HLB. [Method] The diversity of cultivable endophytic bacteria in different samples of D. citri with or without Ca. L. Asiaticus from different geographical origins were compared by traditional isolation and culture methods. D. citri with and without Ca. L. asiaticus were divided into three parts：the head, thorax and abdomen, respectively. Then V6-V8 area of 16S rDNA were amplified after extracting DNA, the endophytic bacteria in D. citri of with or without HLB pathogen and in different parts of the same carrier state were compared by 16S rDNA-PCR-DGGE. The relative amount in 8 D. citri samples of Bacillus sp., Salmonella sp., Enterobacter sp. and Ca. L. asiaticus was determined, which have different results in traditional isolation and culture methods and PCR-DGGE, using total amount of bacteria as an amount of contrast. Relative amounts of the 3 chosen bacteria and Ca. L. asiaticus, respectively, in every D. citri sample were compared before comparing similarity of the same bacteria in different samples in order to analyze the interaction of the 3 chosen bacteria and Ca. L. asiaticus. [Result] Richness of the colony and colony-forming unit of cultivable endophytic bacteria in D. citri with Ca. L. asiaticus were more than in D. citri without Ca. L. asiaticus. Fourteen kinds of bacteria were isolated from D. citri without Ca. L. asiaticus, belonging to 10 genera, such as Bacillus sp.（3）, Erwinia sp.（1）, Klebsiella sp.（1）, Staphylococcus sp.（2）, Arthrobacter sp.（1）, Pantoea sp.（2）, Pectobacterium sp.（1）, Salmonella sp.（1）, Streptomyces sp.（1）, and Massilia brevitalea sp.（1）. While only 4 kinds of bacteria were isolated from D. citri with Ca. L. asiaticus, from 3 genera the same as healthy psyllid, such as Bacillus sp.,