中文摘要：

目的：建立空心莲子草药材的定性定量方法。方法：采用TLC法进行定性鉴别，以三氯甲烷-甲醇（40：1）为展开剂；采用HPLC法测定齐墩果酸的含量，色谱柱为Alltima C18（250mm×4．6mm，5μm），以甲醇-水-冰醋酸-三乙胺（90：10：0．03：0．06）为流动相，流速0．6mL·min^-1，检测波长210nm，柱温25℃。结果：空心莲子草薄层色谱鉴别专属性强；齐墩果酸进样量在1．03～4．99μg范围内与峰面积呈良好的线性关系，线性回归方程为Y=3．676×10^3X-3．958×10^3（r=0．9997），平均回收率（n=6）为99．8％（RSD=2．3％）。结论：本方法简便、准确，重复性好，能有效控制空心莲子草药材质量。

英文摘要：

Objective：To establish qualitative and quantitative methods of Alternanthera philoxerodes. Method： Qualitative analysis for Alternanthera philoxerodes by TLC ;the chromatographic condition for Alternanthera philoxerodes were：a developer of chloroform - methanol （40：1 ）. The content of oleanolic acid in Alternanthera philoxerodes was determined by HPLC ,Alltima C18 colunm（250 mm ×4.6 mm,5 μm）was used. The mobile phase was a mixture of methanol - water - glacial acetic acid - triethylamine （90： 10： 0. 03： 0. 06 ） at a flow rate of 0.6 mL · min^ -1, column temperature was 25 ℃, and the detection wavelength was 210 nm. Results ：Alternanthera philoxerodes could be detected by TLC ; the calibration curve of oleanolic acid was linear over the range of 1.03 - 4.99 μg, and the equation of linear regression was Y = 3. 676 × 10^3X - 3. 958 × 10^3 （ r = 0. 9997 ）. The average recovery rate （ n = 6） was 99.8%, RSD = 2. 3%. Conclusion：The method is convenient, accurate and have a good reproducibility. The quality of Alternanthera philoxerodes can be controlled effectively.