中文摘要：

目的：初步比较麻痹性贝类毒素GTX2，3间接竞争酶免疫学检测方法（idc—ELISA）与直接竞争酶免疫学检测方法（de—ELISA）。方法：用高碘酸盐氧化法制备GTX2，3-HRP偶联物并用直接ELISA对其进行鉴定。分别用GTX2，3与葡萄糖氧化酶（GOX）的偶联物GTX2，3-GOX和兔抗小鼠IgG多抗做为包被抗原，用GTX2，3标准毒素作为抑制剂，做间接及直接竞争ELISA，确定并比较两种ELISA的特异性、灵敏度、检测限、工作范围。结果：成功制备出GTX2，3-HRP偶联物，间接和直接竞争ELISA灵敏度分别为10μg／ml和4μg／ml，检测限为6．25μg／ml和0．5μg／ml，工作范围为6—50μg／ml和0．5—50μg／ml。结论：两种竞争ELISA都能应用于麻痹性贝类毒素的快速检测，但dc—ELISA比idc—ELISA更加灵敏，更适合于进一步的研究。

英文摘要：

Objective：To compare direct and indirect competitive enzyme - linked immunoassay （dc and idc ELISA） for the detection of GTX2, 3 primary. Methods： GTX2,3 was conjugated with HRP, and the conjugates was identified by direct ELISA. GTX2,3 -GOX and rabit anti -mouse IgG polyclonal antibodies were used as coated antigen respectively. Standard toxins were used as inhibitors, idc -ELISA and dc -ELISA were established and compared. Results： GTX2,3 was conjugated with HRP successfully. The sensitivity of dc ELISA and idc ELISA was 10 μg/ml and 4 μg/ml, the detection limits 6, 25 μg/ml and 0. 5 μg/ml, the work range 6 - 50 μg/ml and 0.5 - 50 μg/ml. Conclusion：Both dc and idc ELISA can be used for the detection of GTX, but dc - ELISA is more sensitive and suitable for further research.