中文摘要：

选择同处于泌乳初期的睢宁白山羊6头，于右乳区经过乳导管灌注10μg／kgCpG—ODN，左乳区则灌入等体积的灭菌100μL0．0lmol／LpH7．2磷酸盐缓冲液（PBs）作为对照，灌注后第3d按同等剂量进行二次灌注；次灌注后第2d，分别于左右乳区经乳导管灌注3mL（2×10^9CFU／mL）金黄色葡萄球菌（S．aurcus），于灌注细菌前（0h），灌注后8h，16h，24h，48h和72h分别收集左右乳区乳汁进行检测。临床症状观察显示，乳腺内灌注3mL（2×10^9CFU／mL）的S．aureus能迅速诱导山羊典型的急性乳腺炎症状。组织学观察显示感染S．aureus后72h乳腺腺泡内仍有嗜中性粒细胞（PMN）浸润，但实验乳区明显减少。乳汁S．aureus数同在感染后24h上升至最高，CpG—ODN能显著降低各个时间点乳汁细菌数。乳汁白细胞介素-6（IL-6）水平同在感染后24h上升至最高，CpG-ODN能显著提高感染后24h乳汁IL．6水平。对照和CpG—ODN处理乳区乳汁肿瘤坏死因子-α（TNF-α）水平分别在感染后24h和16h上升至最高，其中在感染后24h实验乳区比对照下降40．63％（P〈0．051。乳汁N-乙酰-β—D-氨基葡萄糖苷酶（NAGase）水平同在感染后16h达最高（P〈0．01），CpG—ODN能显著提高感染后8h乳汁NAGase水平。上述结果表明CpG—ODN可通过提高乳汁IL-6水平、加速并促进乳汁TNF-α的释放，从而减少了乳汁中S．aureus数量，减轻了炎症介质对细胞的损伤，对缩短炎症过程也有一定的作用，实验结果证实了CpG—ODN对S．aureus感染诱发的山羊乳腺炎的乳腺有保护作用。

英文摘要：

The protective effect of CpG-ODN on mastitis was evaluated in Suining white goats with mastitis induced by Staphylococcus aureus Infection. The goat mammary glands were treated with or without CpG-ODN followed by infection with S.aureus, Milk samples and mammary tissues were collected for bacteria counts and histopathologic examinations. The results showed that CpG-ODN treated mammary quarters had much lower bacteria counts in milk than controls at the different time points post-infection. The CpG-ODN could induce significant increase of IL-6 in milk at 24 h post-infection, and induce significant increase of NAGase in milk at 8 h post-infection. These results indicated that CpG-ODN fimctioned as an protective agent against mastitis by stimulating the production of IL-6, prompting the release of the TNF-α, reducing the bacteria counts in milk, attenuating the destruction of inflammation-mediator to cell and shortening the inflammation course.