中文摘要：

目的了解携带供者抗原的第三方树突状细胞（DC）是否具有与供者源未成熟DC相似的免疫功能。方法雌性C57 BL／6小鼠、BALB／c小鼠和昆明小鼠分别为皮肤移植的供者、受者和第三方。将40只BALB／c小鼠分为对照组、环磷酰胺组、供者源未成熟DC组、第三方未成熟DC组、携带供者抗原第三方DC组，每组8只。后4组大鼠皮肤移植术前4d用环磷酰胺（200mg／kg）预处理，对照组同法给予等量等渗盐水。后3组术前2d用1ml相应DC悬液（1×10^7个／m1）预处理，并在术后12d重复给予1mlDC悬液（1×10^7个／m1）1次；前2组于上述2个时相点同法给予等量等渗盐水。记录各组皮片平均成活时间（MST）并于术后5d对皮片进行组织学观察。结果与对照组（16．1±3．5）d比较，供者源未成熟DC组和携带供者抗原第三方DC组小鼠移植皮片的MST明显延长，分别为（38．3±7．7）、（34．9±7．7）d（P〈0．01）；携带供者抗原第三方DC组与供者源未成熟DC组皮片的MST相近（P〉0．05），但与第三方未成熟DC组（23．7±2．7）d比较，差异有统计学意义（P〈0．05）。镜下见携带供者抗原第三方DC组移植皮片结构较清楚、排列有序，与供者源未成熟DC组情况相近。结论携带供者抗原的第三方DC与供者源未成熟DC，均可在一定程度上建立抗原特异性免疫耐受。

英文摘要：

Objective To investigate whether the third-party dendritic cells（DC） incubated with do- nor＇s antigens posess the similar immune functions with donor derived immature DC. Methods Female C57 BL/6, BALB/c and Kunming mice were used as skin transplant donors, recipients and third-party, respectively. Forty BALB/c mice were randomly divided into A （ normal control）, B （ cyclophosphamide administration） , C （ CTX and donor derived immature DC） , D （ CTX and third-party immature DC） , E （ CTX and third-party DC loaded with donor＇s antigens） groups, with 8 mice in each group. The mice in group B, C, D and E were given CTX（200mg/Kg） 4 days before operation, while those in group A were given equivalent amount of normal saline（NS）. Then in group C ,D and E, DC at a dose of 1 × 10^7/ml were intraperitoneally injected with 2 days before grafting and 12 days after operation, but the mice in group A and B were given NS in the same manner. Mean survival time （MST） of skin grafts was recorded, and biopsies of grafts on 5 and 10 post-operation days （POD） were harvested for histologic examination. Results Compared with groupA[（16.1 ±3.5）d],MSTofskin grafts in group C[（38.3 ±7.7）d] and E[（34.9 ±7.7）d] were significantly prolonged （ P 〈 0.01 ） ,while no obvious difference was observed between group C and E（ P 〉 0. 05 ）, but there was statistically significant difference in MST between group D [ （23.7 ± 2.7） d ] and E （ P 〈 0. 05）. In addition, clear epithelial structure and infiltration of inflammatory cells were observed in specimens from both groups C and E. Conclusion Both donor derived immature DC and third-party DC loaded with donor＇s antigens can partly induce donor specific transplantation tolerance.