中文摘要：

试验以天女木兰叶芽、幼叶、成熟叶、花芽、花瓣、种子为材料，分别采用Trizol法、改良Trizol法、CTAB-异丙醇法、CTAB-NaAc和无水乙醇法、CTAB-LiCl法、天根试剂盒法对其各器官进行总RNA的提取。总RNA的完整性、纯度和浓度等提取效果分别通过琼脂糖凝胶电泳和酶标仪进行检测。结果表明：叶芽的总RNA最适合用改良Trizol法进行提取，花瓣和花芽的总RNA最适合用改良的CTAB法提取，幼叶的总RNA可选择试剂盒法，Trizol可用于成龄叶片的提取，而天根试剂盒最适合天女木兰种子总RNA的提取。通过不同提取方法获得的高质量总RNA经RT-PCR检测表明：可以直接用于后续分子克隆和基因表达分析等分子生物学实验。

英文摘要：

Six extraction methods, including modiifed CTAB, Trizol, modiifed Trizol, TIANGEN extraction kit, were studied in the RNA extraction from Magnolia sieboldii buds, leaves, mature leaves, flower buds, petals and seeds. Integrity, purity and concentration of total RNA were examined by agarose gel electrophoresis and nucleic acid analyzer. Of these six methods, the method of modiifed Trizol was better for buds isolation, the method of modiifed CTAB was better for lfower buds and petals isolation, Trizol method can be used to extract mature leaves, while the RNA extracted by TIANGEN kit is effective to the leaves and seeds. High quality total RNA obtained by different extraction methods through RT-PCR analysis indicated that the RNA satisifed the standard for gene cloning and other molecular biological experiments.