中文摘要：

目的研究S100A4在肺鳞癌与肺腺癌细胞系中的表达及对细胞增殖、转移、侵袭功能的影响。方法利用RT-PCR和Western Blot检测S100A4在肺鳞癌细胞系NCI-H520和肺腺癌细胞系SPC-A-1中的表达,利用siRNA干扰S100A4在两个细胞系的表达,CCK-8检测细胞增殖,划痕、Transwell实验检测细胞迁移侵袭。结果RT-PCR与Western Blot检测结果显示S100A4在肺鳞癌及肺腺癌细胞系中均有表达,且肺鳞癌细胞系NCIH520中S100A4表达水平低于肺腺癌细胞系SPC-A-1（P〈0.05）。细胞荧光、Western Blot及RT-PCR验证S100A4敲除效率,CCK-8实验证明干扰S100A4后,两肺癌细胞系的增殖能力较空白对照组和阴性对照组均降低（P〈0.05）、划痕及Tranwell实验证明干扰S100A4后,两肺癌细胞系的迁移能力与对照组相比也降低（P〈0.05）。结论 S100A4在肺鳞癌细胞系与肺腺癌细胞系均有表达,且鳞癌高于腺癌,S100A4与肺癌细胞恶性进展有关,有可能成为肺癌治疗的新靶点。

英文摘要：

Objective To explore the expression of S100A4 in lung squamous carcinoma and adenocarcinoma cells and its effection in proliferation, metastasis,invasion. Methods RT- PCR and Western Blot were used to detect the expression of S100A4 in lung squamous carcinoma NCI - H520 and adenocarcinoma SPC - A - 1 cell lines, using siRNA to knockdown S100A4 in these two cell lines and CCK -8 ,wound healing assay and transwell experiment were used to detect the cell proliferation, migration and invasion. Results The expression of S100A4 was different in these two cell lines and the expression was lower in squamous carcinoma than adenocarcinoma （ P 〈 0.05 ）. The transfection efficiency was detected by immunofluorescence,Western Blot and RT- PCR. Knocking down S100A4 significantly reduced the cell proliferation, migration which was confirmed by CCK - 8, wound healing and transwell assay （ P 〈 0.05 ）. Conclusion Expression of S100A4 in lung squamous carcinoma NCI - H520 was significantly lower than adenocarcinoma SPC - A - 1 cell line. Knocking down S100A4 reduced the lung cancer cell malignant and may be a potential target for lung cancer treatment.