中文摘要：

优化建立鲤鱼（cyprinus carpio,CC）嗅觉端脑（嗅脑）全蛋白提取技术。联用低渗透裂解和液氮冻溶法破碎鲤鱼嗅脑组织（rhinencephalon tissue of cyprinus carpio,CCRT）、低速离心提取CCRT全蛋白,并采用双向凝胶电泳（2D-PAGE）技术进行有效分离。经分析与统计,每张CCRT的2D-PAGE图谱中的蛋白质斑点数目约为1200个。分别分离CCRT的脂溶性和水溶性全蛋白,并获得高分辨率的2D-PAGE图谱。选用差异蛋白质组学技术筛选经10%冰醋酸创伤后的CC,其端脑组织所表达出的6种应激蛋白质,并用肽质量指纹谱（peptide mass fingerprinting,PMF）和数据库检索技术给予鉴定。其中3种蛋白质为70S热休克蛋白、β微管蛋白和DNA链接酶IV,有望作为研究大脑急性创伤后的应激修复途径和机理的指示蛋白质。

英文摘要：

A combined available approach of low osmosis and freeze-soluble with liquid nitrogen was employed to disrupt the rhonencephalon tissue of Cyprinus carpio（CC）. A centrifugal method with low speed was used to extract proteins in rhinencephalon tissue of CC （CCRT）, and high-resolution 2D-PAGE map with 1200 protein spots was obtained. In this study, several techniques were employed for extracting and separating proteome in CCRT. Three lysis solutions were prepared to disrupt the tissue in the fish, and used to extract proteom, water-soluble proteins and fat-soluble proteins, respectively. Both fat-soluble and water-soluble proteins from the CCRT were effectively separated by an optimized 2D-PAGE method with high resolution. With treating by 10% acetic acid, six acute proteins in CCRT were selected by proteomic techniques, and further identified by peptide mass fingerprinting （PMF） and database research, respectively. Three of these differen- tial proteins can be primarily defined to be 70 kD heat-shock protein, DNA ligase IV, and β 5 tubulin. It was proposed that these proteins may be used as markers to study the repairing pathway and mechanism in brain once wounded.