中文摘要：

目的：从补肺益肾方药对脂多糖（LPS）刺激细胞分泌细胞因子变化的影响，揭示补肺益肾方药治疗COPD分子机理。方法：LPS刺激肺泡上皮细胞A549、气道上皮细胞H292、单核巨噬细胞THP-1，每种细胞均分为正常组（20％aY-常大鼠血清）、LPS组（20％正常大鼠血清和75Pg·mL。LPS）、用药组（20％补肺益肾方药大鼠血清和75Pg-mL。LPS），ELISA法检测细胞因子，应用网络工具分析细胞因子的转录因子。结果：与正常组比较，LPS组THP-1细胞分泌MMP-9、TGF—B增多，分泌TNF-α、PPAP2B减少；A549细胞分泌TIMP增多，分泌IL-3、TGF—β减少；H292细胞分泌IL-8增多。与LPS组比较，用药组THP-1细胞分泌TNF-a、TGF—β、TIMP—1、MMP-9（P〈0．01）及GM—CSF、SOD、CD36（P〈0．05）增加，TGF—β分泌减少（P〈0．01）；H292细胞分泌IL-8减少（P〈0．01），TNF-α、TIMP-1、PPAP2B（P〈0．05）和TGF—β（P〈0．05）分泌增加；A549细胞分泌TIMP-1、TNF-α（P〈0．01）和PPAP2B（P〈0．05）减少，MMP-9、IL1B增加（P〈0．01）。与LPS组比较，用药组有改变细胞因子的转录因子：THP—1细胞的AP1、NFKB1、STAT3、SMAD3和PPARG，A549细胞的STAT3和PPARG满足激活条件。结论：LPS对单核巨噬细胞THP-1分泌细胞因子影响最大，补肺益肾含药血清对LPS引起的细胞因子分泌变化有一定调节作用。

英文摘要：

This study was aimed to observe effect of invigorating lung-kidney prescription （ILKP） on secretion of cytokines induced by LPS in vitro and reveal molecular mechanism of ILKP treatment on chronic obstructive pulmonary disease （COPD）. Alveolar epithelial cells A549, airway epithelial ceils H292, and monocyte macrophage THP-1 were stimulated with lipopolysaccharide （LPS）. Samples were divided into the normal group （normal rat serum）, LPS group （20% normal rat serum and 75 pg. mL-1 LPS）, and ILKP group （20%ILKP serum and 75 pg. mL-1 LPS）. The cytokines were detected by ELISA and the transcription factors of cytokines were analyzed with the Network tool. The results showed that compared with the normal group, the secretion of MMP-9 and TGF-β by THP-1 cells, secretion of TIMP by A549 cells, and secretion of IL-8 by H292 cells increased, while secretion of TNF-α, PPAP2B by THP-1 cells, and secretion of IL-3, TGF-β by A549 cells decreased in LPS group. Compared with LPS group, the secretion of TGF-β by THP-1 cells, and secretion of TIMP-1, TNF-α and PPAP2B by A549 cells, and secretion of IL-8 by H292 cells decreased, while secretion of TNF-α, TGF-β, TIMP-1, MMP-9 （P 〈 0.01） and GM-CSF, SOD, CD36 （P 〈 0.05） by THP-1 cells, secretion of MMP-9, IL1B by A549 cells, and secretion of TNF-α, TIMP-1, PPAP2B and TGF-β by H292 cells increased in ILKP group. Compared with LPS group, the transcription factors of cytokines changed in ILKP group, AP1, NFKB1, STAT3, SMAD3 and PPARG in THP-1 cells, STAT3 and PPARG in A549 cells met activation criteria. It was concluded that the THP-1 cells cytokines secretion is the most affected by LPS. The ILKP prescription serum can regulate cells secretion of cytokines induced by LPS in some degree.