采用本课题组建立的定量方法研究了中温内循环厌氧反应器中絮状污泥的成核过程.经过85 d,污泥平均粒径由47.8μm增至96.1μm,成核过程基本完成.成核过程中的污泥平均粒径与成核时间线性相关性显著,相关系数达到0.989 3,污泥粒径的平均增长速率为0.58μm/d.污泥含核率从第1 d的7.6%增长至第85 d的36.1%.含核率的增长速率波动较大,经历了快-慢-较快3个阶段.试验过程中,污泥ECP含量与污泥含核率的增长速率明显正相关,表明ECP可能是污泥成核速率波动的原因.随成核进行污泥活性呈上升趋势,而污泥沉降性能的改善并不明显.上述定量方法以及研究结果均有利于在反应器层面了解污泥颗粒化机制.
The nucleation of flocculent sludge as seed in a mesophilic Internal Circulation Anaerobic reactor was investigated with a quantitative method proposed by us in this paper. In the 85^th days, the average sludge diameter increased from 47.8 μm to 96.1 μm, which indicated that the nucleation was completed. During this process, the average sludge diameter had a significant linear correlation with the time with coefficient of 0.989 3, the increase rate of average sludge diameter was 0.58 μm/d. Nucleus ratio of sludge increased from 7.6% on the 1^st day to 36.1% on the 85^th day. The increase rate of the nucleus ratio fluctuated in a wide range, experienced ' fast-slow-fast' three stages. During the experiment, the concentration of extra cellular polymers (ECPs) of the sludge was significantly positively correlated with the increase rate of the nucleus ratio, which indicated that ECPs could affect nucleation rate. During nucleation, the bio-activity of the sludge increased, but the improvement of settlement ability was not significant. The above-mentioned quantitative method and results would be useful to understand the sludge granulation mechanism on the level of reactor.