中文摘要：

鳃暴露在水环境中,增加了对疾病的易感性。为了研究稀有鮈鲫人工感染草鱼呼肠孤病毒过程中鳃部先天性免疫反应机制,我们克隆了抗病毒效应分子Mx基因的部分序列,用适时荧光定量PCR检测双链RNA的模式识别受体（Toll-like receptor3,TLR3）及I型干扰素指示基因Mx的表达。TLR3和Mx基因的表达在注射病毒后12h显著升高（p〈0.05）,TLR3的表达水平在注射后48h恢复到正常水平（p〉0.05）,而Mx的高水平表达一直持续到实验结束（p〈0.05）。结果表明在GCRV感染中,鳃能发生局部免疫反应,其干扰素途径被激活。

英文摘要：

Exposure to the external aqueous environment enhances the susceptibility to infectious diseases in fish gills.To understand the mechanism of local innate immunity against infectious grass carp reovirus（GCRV） in the gills,we identified the partial cDNA sequence of Mx,an antiviral effector molecule,in rare minnow Gobiocypris rarus.Real-time quantitative RT-PCR（qRT-PCR） was employed to quantitate mRNA levels of a pattern recognition receptor,Toll-like receptor 3（TLR3）,and type I interferons indicator molecule Mx.The artificial infection experiments revealed that TLR3 and Mx mRNA expressions were simultaneously significant up-regulation at 12h postinjection（p〈0.05）,the transcription level of TLR3 recovered at 48h postinjection（p〉0.05）,and the Mx mRNA expression kept at high transcription level till moribund（p〈0.05）.These results highlighted the importance of the gills as a tissue capable of mounting a local immune response,and the interferon pathway was activated to GCRV infection.