中文摘要：

目的：研究4株真眼点藻（Eustigmatos sp．、Eustigmatos polyphem、Vischeria helvetica、Nannochloropsis oculata）的生长、色素组成及光合生理特性。方法：采用重量法、HPLC和分光光度法及液相氧电极法测定藻细胞的色素组成和光合生理指标在不同时相的变化。结果：4种藻最大生物质浓度分别为：9．4g·L-1（E．sp．）、10．42g·L。（E.polyphem）、7．26g·L-1（V.helvetica）和7．15g·L-1（moculata）；随培养时间延长，其chlorophylla／β-carotene和violaxanthin／β—carotene的值均降低，最大光合速率先上升后下降，最大呼吸速率则不断上升；N.oculata的最大光合速率和呼吸速率达到最高，分别为59．62μmol O2·mg～Ch／a·h。和54．23μmol O2·mg-1 Chla·h～；4种藻的77K低温荧光发射光谱均没有高等植物PS I 730nm处的特征峰。结论：真眼点藻的生长与光合生理特征具有种间差异性，其77K低温荧光发射光谱与高等植物相比具有特异性。

英文摘要：

Objective： Determination of the growth, pigment composition and photosynthetic characteristics of four eustigmatophycean spe- cies （ Eustigrnatos sp. , Eustigmatos polyphem, Vischeria helvetica, Nannochloropsis octtlata）. Method： The gravimetric method, HPLC, spectrophotometry and liquid oxygen electrode method were used to measure the pigment composition, photosynthetic physiological charac- teristics of algae cells in different growth phases. Result： The maximum biomass concentrations of four eustigmatophycean species were 9.4 g · L-1 （E. sp. ）, 10. 42 g · L-1 （E. polyphern）, 7. 26 g · L-1 （ V. helvetica） and 7.15 g L-1 （N. oculata）, respectively. The ratio of chlorophyll a / β - carotene and violaxanthin / β - carotene were declining along with the growth phase, the maximal photosynthetic rate went up first and dropped after, while the respiration rate were raising, the largest photosynthetic and respiration rate reached the summit in N. oculata, were 59. 62 and 54. 23 lμmol O2·mg - 1 Chla · h - 1 , respectively. 77K low - temperature fluorescence emission spectra were similar among the four species, but lacked the PSI characteristic peak of the higher plant in 730 nm. Conclusion： The growth and photo- synthetic physiological characteristics were different among the four eustigmatophycean species, and their 77K low - temperature fluores- cence spectra were distinctive to the higher plant.