中文摘要：

果树果实的成熟期与保鲜是影响其经济价值的重要因素,ACC氧化酶是植物乙烯合成途径中的重要酶,催化ACC合成乙烯,进而催化果实的成熟.研究ACC氧化酶基因（ACO）对标记和提高果实的保鲜力具有重要意义.本研究对中华猕猴桃（Actinidia chinensis）、美味猕猴桃（Actinidia deliciosa）、杏（Prunus armeniaca）、葡萄（Vitis vinifera）、苹果（Malus domestica）、西洋梨（Pyrus communis）、沙梨（Pyrus pyrifolia）、桃（Prunus persica）、梅（Prunus mume）、大蕉（Musa ABB）10种果树的ACO基因进行氨基酸序列、蛋白质理化性质及结构、系统发育等生物信息学进行分析,并对这10种果树进行聚类.结果表明：（1）苹果较梅更耐储藏,桃与杏保鲜能力相当,西洋梨和沙梨保鲜力相当,中华猕猴桃和美味猕猴桃保鲜力相当;（2） ACO基因可用于同科物种间保鲜能力的选择,这可能与ACO基因编码氨基酸序列18位点处变异、2OG-Fell Oxy基因家族的高度保守和ACO的多元作用机制有关;（3） ACO的作用机制为在游离核糖体上合成稳定的亲水性蛋白质后,不经蛋白转运,直接在细胞膜内表面氧化ACC合成乙烯,受基因型和外界刺激的多重影响.因此,果树ACO基因可用于属间物种间果实保鲜能力的选择,是一个重要的功能基因.

英文摘要：

Fruits preservation and fructescence were important factors that influenced the economic value of fruits. ACC Oxidase （ACO） was an important enzyme and played a crucial role in regulating fruit growth. To study the mechanism that ACO catalyzed ACC to ethylene might be contributed to mark and improve fruits preservation. Fruit ACO genes data was derived from Actinidia chinensis, Actinidia deliciosa , Vitis vinifera , Malus domestica , Pyrus communis , Pyrus pyrifolia , Prunus persica , Prunus mume, Prunus armeniaca, and Musa ABB. Several aspects of bioinformatics analysis, such as amino acids sequences analysis and chemical properties analysis and phylogenic prediction of them, were conducted. Those fruit trees were grouped together by cluster analysis based on the bioinformatics analysis results. The results of this study were as follows： （1） The fruits preservation of M. domesticaP. Communis and P. pyrifolia had the equal fruit preservation capability. A. chinensis and A. deliciosa had the equal fruit preservation capability. （2） According to the fruits preservation, the plants in a common family could be clustered together by the ACO genes. This might be related to variations on the 18th locus in ACO protein sequence, and highly conserved regions in the 2OG-FeII Oxy superfamily, and ACO mechanism affected by several factors. （3） The mechanism of ACO that the hydrophilic ACO protein synthesized by free ribosomes, directly catalyzed ACC to ethylene in the membrane surface, might be affected by genotype and the outside stimulation sensitivity. The ACO gene was an important function gene： the ACO genes could mark the fruits preservation of the plants among common genus.