中文摘要：

目的观察溶酶体4次穿膜蛋白β（LAPTM4β）在小鼠卵母细胞及早期胚卵中的表达,分析新基因LAPTM4β在植入前胚卵发育中的作用,为研究植入前胚卵发育的基因调控提供实验依据。方法从50只小鼠的子宫或输卵管中收集小鼠卵母细胞及从原核期到胚泡的早期胚卵共50枚。采用激光扫描共焦显微镜技术与免疫组织化学ABC法,观察小鼠卵母细胞及早期胚卵中LAPTM4β的表达;表达结果用Maticam2206图像分析系统进行半定量分析。结果激光扫描共焦显微镜下显示,小鼠卵母细胞和原核期胚卵细胞质的绿色荧光呈不均匀分布,近细胞膜处荧光信号强;2细胞期、4细胞期、8细胞期和桑椹胚卵裂球胞质中绿色荧光均呈局灶性分布,且靠近细胞膜处胞质中绿色荧光均较强,2细胞期中所见极体内也有绿色荧光信号;小鼠胚泡滋养层细胞及内细胞团中均可见中等强度的绿色荧光。免疫组织化学显示,在小鼠卵母细胞中可见LAPTM4β阳性反应颗粒呈浅棕色;原核期、2细胞期、4细胞期、8细胞期胚及桑椹胚,可见深棕色颗粒,且在卵裂球胞质中呈不均匀分布;胚泡的内细胞团及滋养层细胞中均可见棕色阳性反应颗粒。图像分析结果显示,卵母细胞与原核期胚相比,8细胞期与桑椹胚相比,桑椹胚与胚泡相比,LAPTM4β的表达量有显著差异（P〈0.05）。结论LAPTM4β在正常小鼠卵母细胞及植入前各期胚卵中均有表达,且具有规律性;不同阶段早期胚卵中LAPTM4β的表达量均高于卵母细胞,推测LAPTM4β与小鼠早期胚卵的细胞分裂、增殖、分化及胚胎发育相关。

英文摘要：

Objective To observe the expression of LAPTM4β in mouse oocytes and preimplantation embryos,and to understand the effect of LAPTM4β in the development process of embryos,which would thus contributes to the study of gene regulation in the developmental process of embryos. Methods Totally 50 oocytes and early embryos were collected from the uteruses or uterine tubes of 50 mice. The expression and intracellular localization of LAPTM4β in mouse oocytes and preimplantation embryos were studied by confocal laser scanning microscopy（CLSM）technique and immunohistochemical staining method,using the Maticam2206 image analysis system as a semi-quantitative method. Results 1. CLSM technique：In the oocytes and pronuclear stage zygotes,LAPTM4β distributes non-uniformly and had a stranger expression near the membrane. In the embryos of 2-cells,4-cells or 8-cells stage and the morula stage,the expression of LAPTM4β in blastomeres cytoplasm was contagious distribution,and it was also stronger near the membrane and the polar body. There were LAPTM4β positive expressions in the trophoblast cells and inner cell mass of blastocysts. 2. Immunohistochemistry：Dilute brown granules have been seen in the oocytes. There were deep brown granules in the embryos of 2-cells,4-cells,8-cells stage and the morula,and brown granules in the trophoblast cells and inner cell mass of blastocysts. 3. Iimage analysis system：The expression of LAPTM4β had significant differences between oocytes and the pronuclear stage zygotes,8-cells stage and morulas,morulas and blastocysts （P〈0.05）. Conclusion LAPTM4β is expressed in mouse oocytes and preimplantation embryos and has regularity. It is also suggested that there is a relationship between LAPT4β and the proliferation and differentiation of early embryos in mice.