中文摘要：

目的 探究白介素（IL）-18在颞下颌关节炎中的作用.方法 2015年11月—2016年4月,30只SPF级雄性BALB/c小鼠适应性喂养1周后,采用随机数字表法分为A、B、C、D、E组,每组6只.B组-E组在第1、21天时分别注射Ⅱ型胶原酶;从第1天开始,A组仅注射0.9%氯化钠溶液,B组注射0.9%氯化钠溶液,C组注射IL-18,D组注射IL-18结合蛋白（IL-18BP）,E组注射IL-18、IL-18BP.第25天,采集各组小鼠外周血、组织灌洗液,采用流式细胞术检测外周血单核细胞/颞下颌关节组织灌洗液巨噬细胞中白介素18受体（IL-18R）阳性表达率,ELISA法检测外周血血浆/颞下颌关节组织灌洗液上清液中肿瘤坏死因子（TNF）-α、IL-1β水平.结果 B组、C组小鼠外周血单核细胞中IL-18R阳性表达率大于A组（P〈0.05）;C组小鼠外周血单核细胞中IL-18R阳性表达率大于B组、D组、E组（P〈0.05）.B组-E组小鼠颞下颌关节组织灌洗液巨噬细胞中IL-18R阳性表达率大于A组（P〈0.05）;C组小鼠颞下颌关节组织灌洗液巨噬细胞中IL-18R阳性表达率大于B组、D组、E组（P〈0.05）.B组-E组小鼠外周血血浆中TNF-α、IL-1β水平高于A组（P〈0.05）;C组小鼠外周血血浆中TNF-α、IL-1β水平高于B组、D组、E组（P〈0.05）.B组-E组小鼠颞下颌关节组织灌洗液上清液中TNF-α、IL-1β水平高于A组（P〈0.05）;C组小鼠颞下颌关节组织灌洗液上清液中TNF-α、IL-1β水平高于B组、D组、E组（P〈0.05）;D组小鼠颞下颌关节组织灌洗液上清液中TNF-α水平低于B组（P〈0.05）;E组小鼠颞下颌关节组织灌洗液上清液中TNF-α水平高于D组（P〈0.05）.结论 IL-18能通过其受体IL-18R刺激单核细胞/巨噬细胞产生TNF-α与IL-1β等炎性因子,从而参与颞下颌关节炎的发生发展,推测IL-18R可能成为颞下颌关节炎的治疗靶点.

英文摘要：

Objective To explore the role of interleukin - 18 （IL-18）in temporomandibular joint arthritis. Methods This study was conducted from November 2015 to April 2016. After being adaptively fed for 1 week,30 male BALB/ c mice （SPF grade）were randomized into 5 groups （group A,group B,group C,group D and group E）with 6 mice in each. Group B, group C,group D and group E were injected collagenase Ⅱ on the 1st and 21st days of intervention,respectively. From the 1st to the 24th days of intervention,group A and group B were injected 0. 9% sodium chloride solution,group C,group D and group E were injected IL-18,IL-18 binding protein （IL-18BP）,IL-18 and IL-18BP,respectively. On the 25th day of intervention, peripheral blood and temporomandibular joint lavage fluid of mice were collected. The positive expression rates of the interleukin -18 receptor （IL-18R）in monocytes in peripheral blood and macrophages in temporomandibular joint lavage fluid were detected by flow cytometry （FCM）. The levels of TNF-α and IL-1β in peripheral plasma/ liquid supernatant of temporomandibular joint lavage fluid were detected by enzyme - linked immunosorbent assay （ELISA）. Results The positive expression rate of IL-18R in peripheral blood monocytes in group B and group C was significantly higher than that in group A （P 〈 0. 05）;the positive expression rate of IL-18R in peripheral blood monocytes in group C was significantly higher than that in group B,group D and group E （P 〈 0. 05）. The positive expression rate of IL-18R in macrophages in temporomandibular joint lavage fluid in group B, group C,group D and group E was higher than that in group A （P 〈 0. 05）;the positive expression rate of IL-18R in macrophages in temporomandibular joint lavage fluid in group C was higher than that in group B,group D and group E （P 〈0. 05）. The levels of TNF-α and IL-1β in peripheral plasma in group B,group C,group D and group E were higher than those in group A （P 〈 0. 05）;the levels of TNF-α and