中文摘要：

试验旨在对环江香猪仔猪腹泻抗性/易感的黏附素13基因（MUC13）单核苷酸多态性（single nucleotide polymorphism,SNP）/单倍型与仔猪腹泻的相关性进行分析。试验采用高分辨率熔解曲线（high resolution melting,HRM）技术对93头环江香猪腹泻仔猪和95头健康对照仔猪进行基因分型,结合DNA测序对HRM检测结果进行验证,并通过SHEsis在线平台确定其单倍型。结果显示,环江香猪MUC13基因的G224A位等位基因分布频率在腹泻组和对照组间差异显著（P〈0.05）,GG、GA和GG＋GA基因型分别与AA基因型相比差异均显著（P〈0.05）。A112G、T170C和G164C位点各基因型频率和等位基因频率在腹泻组和对照组之间差异均不显著（P〉0.05）,腹泻组与对照组E位点均为野生纯合基因型。单倍型Ⅹ（GTAC）在腹泻组的分布极显著高于对照组（P〈0.01）。因此,MUC13基因G224A位点和单倍型Ⅹ（GTAC）与环江香猪仔猪断奶前腹泻相关。

英文摘要：

The purpose of the study was to analyze the association between MUC1 3 gene polymor-phisms and piglet diarrhea in Huanjiang miniature pigs.93 diarrhea piglets and 95 healthy controls were included in the study.The MUC1 3 gene polymorphisms were determined using high resolu-tion melting （HRM）,and the results of HRM were verified by direct sequencing.Mutations were compared between diarrhea piglets and healthy controls.SHEsis online platform was used to cal-culate the haplotypes.There were statistically significant association on G224A site showed signif-icant difference between diarrhea piglets and healthy controls by allele frequencies （P〈0.05）,and there were also statistically significant differences between the two groups by genotypes among GG,GA,GG＋GA and AA （P〈0.05）.But no statistically significant association was found be-tween polymorphisms of A112G,T170C and G164C sites and piglet diarrhea risk （P〉0.05）. However,haplotype Ⅹ （GTAC）in the distribution of diarrhea group was extremely significantly higher （P〈0.01）.The present data revealed that MUC13 gene G224A site and the haplotypes Ⅹ（GTAC）were associated with reduce risk of piglet diarrhea.