中文摘要：

目的：建立以反相高效液相色谱（RP—HPLC）法测定复肝口服液中大黄素含量的方法。方法：色谱柱为ZorbaxSB—C18（150mm×4．6mm，5μm），流动相为甲醇-0．1％磷酸（85：15），检测波长为430nm，柱温为30℃，流速为1．0mL·min^-1结果：大黄素进样量在0．0752～0．3760mg范围内与峰面积积分值呈良好的线性关系（r=0．99996）；平均回收率为99．77％，RSD=1．03％（n=6）。结论：本方法简便、快速、重现性好，可用于复肝口服液的质量控制。

英文摘要：

OBJECTIVE： To establish a RP - HPLC method for the determination of the content of Emodin in Fugan oral liquid. METHODS： The sample was separated on Zorbax SB- C18 column（150 mm · 4.6 mm, 5 μm） with methanol- 0.1% phosphoric acid solution（85 ： 15） as the mobile phase at a flow rate of 1.0 mL ·min^-1.The detection wavelength was set at 430 nm and the column temperature was 30 12 .RESULTS： The calibration curve was linear at a range of 0.075 2 mg-0.376 0 mg （r = 0.999 96） for the Emodin.The average recovery was 99.77% and the related standard deviation（RSD） was 1.03% （n = 6） .CONCLUSION： This method was proved to be simple, quick and reproducible and can be used for the quality control of Fugan oral liquid.