中文摘要：

采用循环伏安法研究了铜与烟酸和8-羟基喹啉配合物（Cu（Hq）（NA）0.5（Hq=8-羟基喹啉,NA=烟酸））的电化学行为。此外,以鲱鱼精DNA为靶点,采用紫外吸收光谱、DNA粘度滴定和差分脉冲伏安法,从分子水平研究了该配合物与DNA的键合方式。结果发现：配合物的中心Cu2＋在循环伏安图上呈现明显的氧化还原峰,峰电流随扫描速度的增加呈增加趋势,并且与扫描速度成正比。配合物的吸收峰强度随着DNA的加入减色显著,氧化还原峰电流也随之减小,式量电位发生正移;粘度实验结果发现DNA的相对比粘度随配合物的加入而增大。这些结果表明配合物与DNA通过嵌插方式发生作用。

英文摘要：

The electrochemical behavior of the complex of Cu （Hq）（NA）0.5（Hq = 8 hydroxyquinoline, NA-- Nicotinic acid） was studied by cyclic voltammetry. In addition,the interaction between the complex and Herring sperm DNA as biomolecular target was studied by UV spectroscopy, DNA viscosity titration experiment and differential pulse voltammetry（DPV）. The results showed that two pairs of well-defined quasi reversible redox peaks of Cu2＋ of complex have been obtained. The peak current significantly increased with the increase of scanning rate and was proportional to the scanning rate. UV absorption peaks of complex was red-shifted and the intensity was weakened with the addition of DNA. In addition, the peak current decreased significantly and potential moved positively. Viscosity titration experiment vertified that the complex increased the relative viscosity of DNA. All of the above results illustrated that the complex and DNA binded by the mode of intercalation,and it is very important for the design of DNA-targeting drugs in the hereditary engineering.